Efficient Coproduction of Mannanase and Cellulase by the Transformation of a Codon-Optimized Endomannanase Gene from &ITAspergillus niger&IT into &ITTrichoderma reesei &IT
文献类型: 外文期刊
第一作者: Sun, Xianhua
作者: Sun, Xianhua;Xue, Xianli;Li, Mengzhu;Gao, Fei;Hao, Zhenzhen;Huang, Huoqing;Luo, Huiying;Yao, Bin;Su, Xiaoyun;Qin, Lina
作者机构:
关键词: mannanase;cellulase;Trichoderma reesei;feed additive
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )
ISSN: 0021-8561
年卷期: 2017 年 65 卷 50 期
页码:
收录情况: SCI
摘要: Cellulase and mannanase are both important enzyme additives in animal feeds. Expressing the two enzymes simultaneously within one microbial host could potentially lead to cost reductions in the feeding of animals. For this purpose, we codon-optimized the Aspergillus niger Man5A gene to the codon-usage bias of Trichoderma reesei. By comparing the free energies and the local structures of the nucleotide sequences, one optimized sequence was finally selected and transformed into the T. reesei pyridine-auxotrophic strain TU-6. The codon-optimized gene was expressed to a higher level than the original one. Further expressing the codon-optimized gene in a mutated T. reesei strain through fed-batch cultivation resulted in co-production of cellulase and mannanase up to 1376 U.mL(-1) and 1204 U.mL(-1) , respectively.
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