Fast and Selective Two-Stage Ratiometric Fluorescent Probes for Imaging of Glutathione in Living Cells

文献类型： 外文期刊

第一作者： Gong, Deyan

作者： Gong, Deyan;Iqbal, Anam;Qian, Jing;Cao, Ting;Liu, Wei;Liu, Weisheng;Qin, Wenwu;Gong, Deyan;Iqbal, Anam;Qian, Jing;Cao, Ting;Liu, Wei;Liu, Weisheng;Qin, Wenwu;Han, Shi-Chong;Guo, Huichen;Han, Shi-Chong;Guo, Huichen;Iqbal, Anam

作者机构：

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）

ISSN： 0003-2700

年卷期： 2017 年 89 卷 24 期

页码：

收录情况： SCI

摘要： Two fluorescent, m-nitrophenol-substituted difluoroboron dipyrromethene dyes have been designed by nucleophilic substitution reaction of 3,5-dichloro-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY). Nonsymmetric and symmetric probes, that is. BODIPY 1 (with one nitrophenol group at the position 3) and BODIPY 2 (with two nitrophenol groups at the positions 3 and 5) were applied to ratiometric fluorescent glutathione detection. The detection is based on the two-step nucleophilic aromatic substitution of the nitrophenol groups of the probes by glutathione in buffer solution containing CTAB. In the first stage, probe 1 showed ratiometric fluorescent color change from green (lambda(em) = 530 nm) to yellow (lambda(em) = 561 nm) because of monosubstitution with glutathione (I-561nm/I-530nm). Addition of excess glutathione caused the second stage of ratiometric fluorescent color change from yellow to reddish orange (lambda(em) = 596 mu, I-596nm/I-561nm) due to disubstitution with glutathione. Therefore, different concentration ranges of glutathione (from less to excess) could be rapidly detected by the two-stage ratiometric fluorescent probe 1 in 5 min. While, probe 2 shows single-stage ratiometric fluorescent detection to GSH (from green to reddish orange, I-596nm/I-535nm). Probes 1 and 2 exhibit excellent properties with sensitive, specific colorimetric response and ratiometric fluorescent response to glutathione over other sulfur nucleophiles. Application to cellular ratiometric fluorescence imaging indicated that the probes were highly responsive to intracellular glutathione.

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