Two acidic, thermophilic GH28 polygalacturonases from Talaromyces leycettanus JCM 12802 with application potentials for grape juice clarification
文献类型: 外文期刊
第一作者: Li, Yeqing
作者: Li, Yeqing;Xu, Bo;Li, Yeqing;Wang, Yuan;Tu, Tao;Zhang, Duoduo;Ma, Rui;You, Shuai;Wang, Xiaoyu;Yao, Bin;Luo, Huiying
作者机构:
关键词: Talaromyces leycettanus JCM12802;Polygalacturonase;Heterologous expression;Grape juice clarification
期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )
ISSN: 0308-8146
年卷期: 2017 年 237 卷
页码:
收录情况: SCI
摘要: Efficient hydrolysis of pectic materials to sugars requires the synergistic action of endo- and exo-polygalacturonases. Two novel polygalacturonases (exo-TePG28a and endo-TePG28b) were identified in Talaromyces leycettanus JCM12802, overexpressed in Pichia pastoris, and characterized in this report. The specific activities of TePG28a and TePG28b towards polygalacturonic acid were 280 +/- 9 and 25,900 +/- 502 U/mg, respectively. Both enzymes exhibited optimal activities at pH 3.5 and retained highly stable over a broad pH range of 2.0-7.0. Distinct from most fungal polygalacturonases that have low temperature optima, TePG28a and TePG28b were optimally active at 70 degrees C. When treated the grape juice with the enzyme combination (the unit ratio of TePG28a:TePG28b was 1:4), higher pectin-degrading efficiency (up to 140%) was achieved, and light transmittance was improved from 14% to 82%. These favorable enzymatic properties make TePG28a and TePG28b attractive for the applications in the juice industry. (C) 2017 Published by Elsevier Ltd.
分类号:
- 相关文献
作者其他论文 更多>>
-
A highly susceptible hACE2-transgenic mouse model for SARS-CoV-2 research
作者:Liu, Gang;Zhang, Min;Han, Xuelian;Wei, Yuwei;Sun, Yali;Cao, Xiangwen;Wang, Yuan;Li, Min;Zhao, Guangyu;Yin, Qi;Sun, Yansong;Wu, Baolei;Zhang, Cheng;Guo, Zhendong;Zhang, Cheng;Guo, Zhendong;Wang, Yan;Li, Li;Wang, Rongjuan;Li, Yalan;Sun, Yali;Cao, Xiangwen;Zhao, Guangyu;Ke, Yuehua
关键词:ACE2; inflammatory response; lung injury; mouse model; SARS-CoV-2
-
Unveiling Innovative Approaches to Mitigate Metals/Metalloids Toxicity for Sustainable Agriculture
作者:Charagh, Sidra;Hui, Suozhen;Wang, Jingxin;Zhou, Liang;Xu, Bo;Zhang, Yuanyuan;Sheng, Zhonghua;Tang, Shaoqing;Hu, Shikai;Hu, Peisong;Raza, Ali
关键词:
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者:Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词:high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者:Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词:
-
The HOG-pathway related AaOS1 leads to dicarboximide-resistance in field strains of Alternaria alternata and contributes, together with the Aafhk1, to mycotoxin production and virulence
作者:Chen, Changjun;Wang, Xiaoyu;Wei, Lingling;Chen, Bin;Li, Xiujuan;Shi, Haiping;Xie, Shuai;Hu, Hao;Chen, Changjun;Chen, Wenchan;Wei, Lihui;Wang, Xiaoyu
关键词:garlic leaf spot; Alternaria alternata; dicarboximides; mutations; mycotoxin AOH
-
A Two-Stage Leaf-Stem Separation Model for Maize With High Planting Density With Terrestrial, Backpack, and UAV-Based Laser Scanning
作者:Lei, Lei;Lei, Lei;Li, Zhenhong;Li, Zhenhong;Yang, Hao;Xu, Bo;Yang, Guijun;Hoey, Trevor B.;Wu, Jintao;Yang, Xiaodong;Feng, Haikuan;Yang, Guijun;Yang, Guijun
关键词:Vegetation mapping; Laser radar; Point cloud compression; Feature extraction; Agriculture; Data models; Data mining; Different cultivars; different growth stages; different planting densities; different platforms; light detection and ranging (LiDAR) data; simulated datasets; two-stage leaf-stem separation model
-
Anthocyanin gene enrichment in the distal region of cotton chromosome A07: mechanisms of reproductive organ coloration
作者:Zheng, Liuchang;Zheng, Liuchang;Zhang, Jilong;He, Haiyan;Meng, Zhigang;Wang, Yuan;Guo, Sandui;Liang, Chengzhen
关键词:cotton; anthocyanin; MYB; GST; metabolic gene clusters