The Cap Snatching of Segmented Negative Sense RNA Viruses as a Tool to Map the Transcription Start Sites of Heterologous Co-infecting Viruses

文献类型： 外文期刊

第一作者： Lin, Wenzhong

作者： Lin, Wenzhong;Qiu, Ping;Jin, Jing;Liu, Shunmin;Ul Islam, Saif;Zhang, Jie;Du, Zhenguo;Wu, Zujian;Yang, Jinguang;Kormelink, Richard;Du, Zhenguo;Wu, Zujian

作者机构：

关键词： cap-snatching;sNSV;tenuivirus;TSS;geminivirus;rice stripe virus;co-infection

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Identification of the transcription start sites (TSSs) of a virus is of great importance to understand and dissect the mechanism of viral genome transcription but this often requires costly and laborious experiments. Many segmented negative-sense RNA viruses (sNSVs) cleave capped leader sequences from a large variety of mRNAs and use these cleaved leaders as primers for transcription in a conserved process called cap snatching. The recent developments in high-throughput sequencing have made it possible to determine most, if not all, of the capped RNAs snatched by a sNSV. Here, we show that rice stripe tenuivirus (RSV), a plant-infecting sNSV, co-infects Nicotiana benthamiana with two different begomoviruses and snatches capped leader sequences from their mRNAs. By determining the 5 ' termini of a single RSV mRNA with high-throughput sequencing, the 5 ' ends of almost all them RNAs of the co-infecting begomoviruses could be identified and mapped on their genomes. The findings in this study provide support for the using of the cap snatching of sNSVs as a tool to map viral TSSs.

分类号：

  • 相关文献

[1]Co-Infection with Marek's Disease Virus and Reticuloendotheliosis Virus Increases Illness Severity and Reduces Marek's Disease Vaccine Efficacy. Sun, Guo-Rong,Zhang, Yan-Ping,Zhou, Lin-Yi,Lv, Hong-Chao,Zhang, Feng,Li, Kai,Gao, Yu-Long,Qi, Xiao-Le,Cui, Hong-Yu,Wang, Yong-Qiang,Gao, Li,Pan, Qing,Wang, Xiao-Mei,Liu, Chang-Jun. 2017

[2]Porcine bocaviruses: genetic analysis and prevalence in Chinese swine population. Zhang, H. B.,Huang, L.,Lin, T.,Sun, C. Q.,Deng, Y.,Wei, Z. Z.,Long, J. X.,Yuan, S. S.,Zhang, H. B.,Liu, Y. J.,Lin, T.,Cheung, A. K.. 2011

[3]Establishment of a Multiplex PCR and an Investigation of Co-Infection Rate of WSSV and IHHNV in Penaeid vannamei in Northern of Jiangsu. Chen, Yi-Bing,Gao, Song,Zhou, Jun-Fang,Wan, Xi-He. 2012

[4]Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs. Wang, Jinbao,Yu, Jiang,Wang, Jinbao. 2012

[5]Co-infection of H9N2 subtype avian influenza virus and infectious bronchitis virus decreases SP-A expression level in chickens. Huang, Qi,Wang, Kai,Pan, Ling,Qi, Kezong,Liu, Hongmei,Huang, Qi,Wang, Kai,Chen, Hongjun.

[6]The effects of Mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro PK-15 cells. Wang, Haiyan,Feng, Zhixin,Wu, Yuzi,Wei, Yanna,Gan, Yuan,Hua, Lizhong,Li, Bin,Wang, Xiaomin,Liu, Maojun,Xiong, Qiyan,Shao, Guoqing,Wang, Haiyan,Feng, Zhixin,Shao, Guoqing.

[7]The occurrence of porcine circovirus 3 without clinical infection signs in Shandong Province. Zheng, S.,Wu, X.,Zhang, L.,Xin, C.,Shi, J.,Peng, Z.,Xu, S.,Yu, J.,Sun, W.,Xu, S.,Li, J.,Wang, J.,Zheng, S.,Liu, Y.,Fu, F..

[8]Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Li, Bin,Du, Luping,Xu, Xiangwei,Sun, Bing,Yu, Zhengyu,Feng, Zhixin,Liu, Maojun,Wei, Yanna,Wang, Haiyan,Shao, Guoqing,He, Kongwang,Li, Bin,Yu, Zhengyu,Feng, Zhixin,Liu, Maojun,Wei, Yanna,Wang, Haiyan,Shao, Guoqing,He, Kongwang.

[9]CHINESE SQUASH LEAF CURL VIRUS - A NEW WHITEFLY-TRANSMITTED GEMINIVIRUS. WANG, XF,TIAN, B,CAI, JH. 1995

[10]Genomic Analysis of the Natural Population of Wheat dwarf virus in Wheat from China and Hungary. Liu Yan,Wang Biao,Wu Bei-lei,Wang Xi-feng,Liu Yan,Wu Yuan-hua,Vida, Gyula,Cseplo-Karolyi, Monika. 2012

[11]Tobacco RING E3 Ligase NtRFP1 Mediates Ubiquitination and Proteasomal Degradation of a Geminivirus-Encoded beta C1. Hu, Tao,Bao, Min,Cao, Linge,Song, Fengmin,Zhou, Xueping,Bao, Min,Zhou, Xueping,Zhang, Huawei,Xie, Qi,Shen, Qingtang. 2016

[12]SGS3 Cooperates with RDR6 in Triggering Geminivirus-Induced Gene Silencing and in Suppressing Geminivirus Infection in Nicotiana Benthamiana. Zhou, Xueping,Li, Fangfang,Wang, Yaqin,Zhou, Xueping. 2017

[13]Mimic Phosphorylation of a beta C1 Protein Encoded by TYLCCNB Impairs Its Functions as a Viral Suppressor of RNA Silencing and a Symptom Determinant. Zhong, Xueting,Wang, Zhan Qi,Xiao, Ruyuan,Cao, Linge,Wang, Yaqin,Xie, Yan,Zhou, Xueping,Zhou, Xueping.

[14]The AC5 protein encoded by Mungbean yellow mosaic India virus is a pathogenicity determinant that suppresses RNA silencing-based antiviral defenses. Xu, Xiongbiao,Huang, Changjun,Gu, Zhouhang,Cao, Linge,Hu, Tao,Li, Zhenghe,Zhou, Xueping,Li, Fangfang,Zhou, Xueping,Ding, Ming.

[15]Geminiviruses and their application in biotechnology. Ding Bo,Zhou Xue-ping. 2017

[16]Tomato Yellow Leaf Curl Virus V2 Interacts with Host Histone Deacetylase 6 To Suppress Methylation-Mediated Transcriptional Gene Silencing in Plants. Wang, Bi,Wang, Yaqin,Xie, Yan,Zhou, Xueping,Wang, Bi,Yang, Xiuling,Zhou, Xueping. 2018

[17]Production of transgenic rice new germplasm with strong resistance against two isolations of Rice stripe virus by RNA interference. Ma, Jin,Song, Yunzhi,Wu, Bin,Li, Kaidong,Zhu, Changxiang,Wen, Fujiang,Jiang, Mingsong. 2011

[18]Production of marker-free and RSV-resistant transgenic rice using a twin T-DNA system and RNAi. Jiang, Yayuan,Sun, Lin,Li, Kaidong,Song, Yunzhi,Zhu, Changxiang,Jiang, Mingsong.

[19]Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity. Zhang, C.,Song, Y.,Jiang, F.,Jiang, Y.,Zhu, C.,Wen, F.,Li, G..

[20]Dimeric artificial microRNAs mediate high resistance to RSV and RBSDV in transgenic rice plants. Sun, Lin,Lin, Chao,Du, Jinwen,Song, Yunzhi,Liu, Hongmei,Zhou, Shumei,Wen, Fujiang,Zhu, Changxiang,Jiang, Mingsong.

作者其他论文 更多>>

微信小程序