Manganese influences the expression of fatty acid synthase and malic enzyme in cultured primary chicken hepatocytes

文献类型： 外文期刊

第一作者： Lu, Lin

作者： Lu, Lin;Wang, Meiling;Liao, Xiudong;Zhang, Liyang;Luo, Xugang

作者机构：

关键词： Manganese;Gene expressions;Fatty acid synthase;Malic enzyme;Broiler hepatocytes

期刊名称：BRITISH JOURNAL OF NUTRITION （ 影响因子：3.718； 五年影响因子：4.816 ）

ISSN： 0007-1145

年卷期： 2017 年 118 卷 11 期

页码：

收录情况： SCI

摘要： Two experiments were designed to investigate the effects of Mn source and concentration on the mRNA expression and enzymatic activities of fatty acid synthase (FAS) and malic enzyme (ME) in cultured primary broiler hepatocytes. In Expt 1, primary broiler hepatocytes were treated with 0 (control), 0.25, 0.50 or 0.75 mmol/l of Mn as inorganic manganese chloride (MnCl2.4H(2)O) for 24 and 48 h. In Expt 2, primary broiler hepatocytes were incubated with 0 (control), 0.25 or 0.50 mmol/l of Mn as either manganese chloride or Mn-amino acid chelate for 48 h. The mRNA levels and activities of FAS and ME in the hepatocytes were measured in Expts 1 and 2. The results in Expt 1 showed that only at 48 h mRNA expression levels of FAS and ME in the hepatocytes decreased linearly (P < 0.001) and quadratically (P < 0.02) as supplemental Mn concentrations increased. In Expt 2, compared with the control, Mn supplementation reduced (P < 0.01) the activities of FAS, mRNA expression levels of FAS and ME in the hepatocytes, and the efflux of lactic dehydrogenase to the medium. The supplemental Mn at 0.5 mmol/l showed a lower (P < 0.03) ME mRNA expression level compared with the Mn group at 0.25 mmol/l. However, Mn source and the interaction between Mn source and concentration had no impacts (P > 0.33) on any of the measured cellular parameters. The results suggested that Mn might reduce cell damage and regulate FAS and ME expression at a transcriptional level in primary cultured broiler hepatocytes.

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