A Single-Nucleotide Deletion in the Transcription Factor Gene bcsmr1 Causes Sclerotial-Melanogenesis Deficiency in Botrytis cinerea

文献类型： 外文期刊

第一作者： Zhou, Yingjun

作者： Zhou, Yingjun;Yang, Long;Wu, Mingde;Li, Guoqing;Zhang, Jing;Zhou, Yingjun;Yang, Long;Wu, Mingde;Li, Guoqing;Zhang, Jing;Zhou, Yingjun;Chen, Weidong

作者机构：

关键词： Botrytis cinerea;orange-colored sclerotia;sclerotial melanogenesis;bcsmr1;single-nucleotide deletion

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Botrytis cinerea is an important plant pathogenic fungus with a wide range of host. It usually produces black-colored sclerotia (BS) due to deposition of 1,8-dihydroxynaphthalene melanin in sclerotial melanogenesis. Our previous study (Zhou et al., 2018) reported six B. cinerea isolates producing orange-colored sclerotia (OS) with deficiency in sclerotial melanogenesis. Comparison of ecological fitness (conidia, mycelia, sclerotia), natural distribution, and melanogenesis of selected BS and OS isolates suggests that sclerotia play an important role in the disease cycle caused by B. cinerea. However, the molecular mechanism for formation of the OS B. cinerea remains unknown. This study was done to unravel the molecular mechanism for the sclerotial melanogenesis deficiency in the OS isolates. We found that all the five sclerotial melanogenesis genes (bcpks12, bcygh1, bcbrn1/2, bcscd1) were down-regulated in OS isolates, compared to the genes in the BS isolates. However, the sclerotial melanogenesis-regulatory gene bcsmr1 had similar expression in both types of sclerotia, suggesting the sclerotial melanogenesis deficiency is due to loss-of-function of bcsmr1, rather than lack of expression of bcsmr1. Therefore, we cloned bcsmr1 from OS (bcsmr1(OS)) and BS (bcsmr1(BS)) isolates, and found a single-nucleotide deletion in bcsmr1(OS). The single-nucleotide deletion caused formation of a premature stop codon in the open reading frame of bcsmr1(OS), resulting in production of a 465-aa truncated protein. The transcription activity of the truncated protein was greatly reduced, compared to that of the 935-aa full-length protein encoded by bcsmr1(BS) in the BS isolates. The function of bcsmr1(OS) was partially complemented by bcsmr1(BS). This study not only elucidated the molecular mechanism for formation of orange-colored sclerotia by the spontaneous mutant XN-1 of B. cinerea, but also confirmed the regulatory function of bcsmr1 in sclerotial melanogenesis of B. cinerea.

分类号：

  • 相关文献

[1]Diversity and biocontrol potential of endophytic fungi in Brassica napus. Zhang, Jing,Yang, Long,Jiang, Daohong,Li, Guoqing,Zhang, Qinghua,Zhang, Jing,Yang, Long,Jiang, Daohong,Li, Guoqing,Zhang, Lei,Chen, Weidong. 2014

[2]Inhibitory effect of boron against Botrytis cinerea on table grapes and its possible mechanisms of action. Qin, Guozheng,Zong, Yuanyuan,Tian, Shiping,Zong, Yuanyuan,Chen, Qiling,Hua, Donglai. 2010

[3]Functional analysis of BT4 of Arabidopsis thaliana in resistance against Botrytis cinerea. Hao, Cong-Cong,Jia, Jiao,Xing, Ji-Hong,Wang, Feng-Ru,Dong, Jin-Gao,Han, Jian-Min,Jia, Jiao,Chen, Zhan,Weng, Qiao-Yun. 2013

[4]Description of Bursaphelenchus taphrorychi sp n. (Nematoda: Parasitaphelenchidae), the second Bursaphelenchus species from larval galleries of the beech bark beetle, Taphrorychus bicolor (Herbst.) (Coleoptera: Curculionidae: Scolytinae), in European beech, Fagus sylvatica L.. Tomalak, Marek,Malewski, Tadeusz,Gu, Jianfeng,Fa-Qiang, Zhan. 2017

[5]Identification of Development and Pathogenicity Related Gene in Botrytis cinerea via Digital Gene Expression Profile. Zhao, Bin,Si, He Long,Sun, Zhi Ying,Xu, Zheng,Zhang, Jin Lin,Xing, Ji Hong,Dong, Jin Gao,Chen, Zhan. 2015

[6]Identification and evaluation of Aspergillus tubingensis as a potential biocontrol agent against grey mould on tomato. Zhao, Juan,Liu, Weicheng,Liu, Dewen,Lu, Caige,Zhang, Dianpeng,Wu, Huiling,Dong, Dan,Meng, Lingling. 2018

[7]The effect of MeJA on ethylene biosynthesis and induced disease resistance to Botrytis cinerea in tomato. Yu, Mengmeng,Shen, Lin,Zhao, Danying,Zheng, Yang,Sheng, Jiping,Fan, Bei.

[8]First report of grey mould on water dropwort (Oenanthe javanica DC.) caused by Botrytis cinerea. Song, Jia-Wei,Shi, Yan-Xia,Xie, Xue-Wen,Chai, A-Li,Li, Bao-Ju.

[9]Defense responses in tomato fruit induced by oligandrin against Botrytis cinerea. Wang, Ai-ying,Lou, Bing-gan,Xu, Tong,Lin, Chai,Wang, Ai-ying. 2011

[10]EARLY DETECTION OF GREY MOULD DEVELOPMENT IN 'RED GLOBE' GRAPES DURING STORAGE. Guan, Wenqiang,Wu, Xinling,Guan, Wenqiang,Wang, Zhidong,Wang, Xiaotuo,Yan, Ruixiang. 2015

[11]Biological control of Botrytis cinerea on tomato plants using Streptomyces ahygroscopicus strain CK-15. Cheng, Y.,Liu, Y.,Liu, B. H.,Zhang, K. C..

[12]A new disease of mung bean caused by Botrytis cinerea. Li, Yinping,Sun, Suli,Xu, Chunbiao,Duan, Canxin,Zhu, Zhendong,Du, Chengzhang,Zhang, Jijun.

[13]BjMYB1, a transcription factor implicated in plant defence through activating BjCHI1 chitinase expression by binding to a W-box-like element. Gao, Ying,Jia, Shuangwei,Wang, Chunlian,Wang, Fujun,Wang, Fajun,Zhao, Kaijun.

[14]Antagonism between phytohormone signalling underlies the variation in disease susceptibility of tomato plants under elevated CO2. Zhang, Shuai,Li, Xin,Sun, Zenghui,Shao, Shujun,Zhou, Yanhong,Xia, Xiaojian,Yu, Jingquan,Shi, Kai,Li, Xin,Yu, Jingquan,Hu, Lingfei,Ye, Meng.

[15]Interkingdom Gene Transfer May Contribute to the Evolution of Phytopathogenicity in Botrytis Cinerea. Zhu, Bo,Zhou, Qing,Xie, Guanlin,Zhang, Guoqing,Li, Bin,Jin, Gulei,Zhu, Bo,Zhou, Qing,Xie, Guanlin,Zhang, Guoqing,Li, Bin,Jin, Gulei,Zhang, Xiaowei,Wang, Yanli,Sun, Gunchang,Jin, Gulei. 2012

[16]Effects of volatile substances of Streptomyces globisporus JK-1 on control of Botrytis cinerea on tomato fruit. Zheng, Lu,Huang, Junbin,Li, Guoqing,Li, Qili,Ning, Ping,Hsiang, Tom. 2012

[17]Molecular mechanism of BjCHI1-mediated plant defense against Botrytis cinerea infection. Gao, Ying,Zhao, Kaijun. 2017

[18]Pyrimethanil sensitivity and fitness in Botrytis cinerea populations from Shanxi Province, China. Ren, Lu,Shi, Xiao-jing,Guo, Ping-yi,Han, Ju-cai,Zhao, Xiao-jun.

[19]Evaluation of Sporidiobolus pararoseus strain YCXT3 as biocontrol agent of Botrytis cinerea on post-harvest strawberry fruits. Huang, R.,Che, H. J.,Zhang, J.,Yang, L.,Jiang, D. H.,Li, G. Q.,Huang, R.,Che, H. J.,Zhang, J.,Yang, L.,Jiang, D. H.,Li, G. Q.,Huang, R..

[20]Inhibitory effect of bionic fungicide 2-allylphenol on Botrytis cinerea (Pers. ex Fr.) in vitro. Gong, Shuangjun,Xia, Yuanyuan,Liu, Xili,Li, Jianqiang,Gong, Shuangjun,Hao, Jianjun.

作者其他论文 更多>>

微信小程序