Evaluation of a quantitative real-time PCR for rapid detection of Riemerella Anatipestifer infection in birds

文献类型： 外文期刊

第一作者： Zhang, Qingshan

作者： Zhang, Qingshan;Li, Chenxi;Bai, Xiaofei;Liu, Ming;Liu, Siguo;Zhang, Yun;Wan, Chunhe

作者机构：

关键词： conventional PCR;TaqMan-based real-time PCR;Riemerella anatipestifer

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2017 年 79 卷 12 期

页码：

收录情况： SCI

摘要： To establish an accurate, rapid, and a quantifiable method for the detection of Riemerella anatipestifer infection, a widespread infectious disease in birds, we developed a TaqMan-based real-time PCR assay by using DtxR gene-specific primers and a TaqMan probe. The standard curve established with a linear correlation (R-2) of 0.998 and efficiency of 99% between the Ct value and the logarithm of the plasmid copy number. The reproducibility and specificity of the real-time PCR assay were confirmed by using plasmids containing DtxR genes or DNAs extracted from well-known bacteria or viruses causing duck diseases. The real-time PCR assay was 100 times more sensitive than the conventional PCR. The results reveal that the established real-time PCR assay might be a useful method for diagnosis and quantitative detection of Riemerella anatipestifer in birds.

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