Screening and separation of alpha-amylase inhibitors from Solanum nigrum with amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography
文献类型: 外文期刊
第一作者: Cen, Yin
作者: Cen, Yin;Chen, Xiaoqing;Xiao, Aiping;Liu, Liangliang
作者机构:
关键词: alpha-amylase;high-speed counter-current chromatography;magnetic graphene oxide;traditional Chinese medicine
期刊名称:JOURNAL OF SEPARATION SCIENCE ( 影响因子:3.645; 五年影响因子:2.943 )
ISSN: 1615-9306
年卷期: 2017 年 40 卷 24 期
页码:
收录情况: SCI
摘要: A screening method using alpha-amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography was proposed and utilized to screen and separate alpha-amylase inhibitors from extract of Solanum nigrum. The alpha-amylase-functionalized magnetic graphene oxide was characterized and found to demonstrate satisfactory structure, magnetic response (24.5 emu/g), and reusability (retained 90% of initial activity after five cycles). The conditions for the screening with alpha-amylase functionalized magnetic graphene oxide were optimized and set at pH 7.0 and 25 degrees C. As a result, two potent flavonoid compounds, apigenin-7-O-glucuronide (1) and astragalin (2), were separated and collected through high-speed countercurrent chromatography and subjected to high-performance liquid chromatography analysis with purity higher than 90% (according to HPLC data), which were identified as alpha-amylase inhibitors. These results suggested that utilization of alpha-amylase functionalized magnetic graphene oxide in the rapid screening and isolation bioactive compounds from complex natural products is a feasible and environmentally friendly method.
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