Expression and inheritance of Bt cry1Ia8 gene in transgenic cabbage to control Plutella xylostella

文献类型： 外文期刊

第一作者： Yi, Dengxia

作者： Yi, Dengxia;Fang, Zhiyuan;Yang, Limei

作者机构：

关键词： Bt cabbage;Agrobacterium tumefaciens-mediated transformation;Progeny analyses;Transgene expression;Transgene inheritance

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2017 年 225 卷

页码：

收录情况： SCI

摘要： We previously transformed cabbage plants with a Bacillus thuringiensis crylIa8 gene. The resulting transgenic plants were able to control both susceptible and CrylAc-resistant Plutella xylostella larvae. For practical applications, transgenes should be inherited in a stable and predictable manner. To evaluate and characterize the stability of transgenic cabbages produced using an Agrobacterium tumefaciens-mediated transformation procedure, transgene expression and inheritance were examined in four single-copy lineages (A14-3, A14-5, A14-7, and A14-8) and their sexually derived progenies. Segregations ratios (insect-resistant:insect-sensitive) of 2.56:1, 2.75:1, 3.24:1, and 1.6:1 were observed in the T-1 generation of lineages A14-3, A14-5, A14-7, and A14-8, respectively. Examinations of CrylIa8 activities in the T-1, T-2, and T-3 plants indicated transgene inheritance followed the Mendelian segregation pattern in lineages A14-3, A14-5, and A14-7. Southern blot and enzyme linked immunosorbent assay analyses of the T-0, T-1, T-2, and T-3 progenies revealed that the transgene was successfully inserted into the cabbage genome, and had been transmitted to self-pollinated T-3 progenies. These results imply that the homozygous transgenic lines exhibiting stable inheritance of the transgene and can be used effectively in breeding programs.

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