Improving cellulase production in submerged fermentation by the expression of a Vitreoscilla hemoglobin in Trichoderma reesei

文献类型： 外文期刊

第一作者： Lin, Jie

作者： Lin, Jie;Zhang, Xiamei;Song, Bingran;Xue, Wei;Chen, Xiuzhen;Dong, Zhiyang;Lin, Jie;Zhang, Xiamei;Song, Bingran;Dong, Zhiyang;Su, Xiaoyun

作者机构：

关键词： Trichoderma reesei;Vitreoscilla hemoglobin;Submerged fermentation;Oxygen limitation;Cellulase

期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）

ISSN： 2191-0855

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Trichoderma reesei is well known as an industrial workhorse fungus in cellulase production. The low dissolved oxygen supply in the highly viscous medium of T. reesei remains a major bottleneck that hampers growth and cellulase production in submerged fermentation. Vitreoscilla hemoglobin (VHb) has been demonstrated to improve metabolism and protein production in different heterologous hosts under hypoxic conditions, but the use of VHb in T. reesei remains uninvestigated. This study examines the effect of VHb in improving T. reesei performance in submerged fermentation. The VHb gene (vgb)-expressing cassette was successfully transformed into the TU-6 strain, integrated into the genome of T. reesei, and functionally expressed with biological activity, which was confirmed by carbon monoxide difference analysis. Compared to the parent strain, the expression of VHb increased the glucose consumption rate of the transformant. Moreover, in cellulase-inducing medium total protein secretion of the VHb expressing strain was 2.2-fold of the parental strain and the filter paper cellulase activity was increased by 58% under oxygen-limiting conditions. In summary, our results demonstrate that VHb has beneficial effects on improving total protein secretion and cellulase activity of T. reesei in submerged fermentation.

分类号：

  • 相关文献

[1]Revisiting overexpression of a heterologous beta-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities. Xue, Xianli,Ma, Rui,Luo, Huiying,Su, Xiaoyun,Yao, Bin,Wu, Yilan,Qin, Xing. 2016

[2]Efficient Coproduction of Mannanase and Cellulase by the Transformation of a Codon-Optimized Endomannanase Gene from &ITAspergillus niger&IT into &ITTrichoderma reesei &IT. Sun, Xianhua,Xue, Xianli,Li, Mengzhu,Gao, Fei,Hao, Zhenzhen,Huang, Huoqing,Luo, Huiying,Yao, Bin,Su, Xiaoyun,Qin, Lina. 2017

[3]Overexpression of Vitreoscilla hemoglobin increases waterlogging tolerance in Arabidopsis and maize. Du, Hewei,Shen, Xiaomeng,Zhang, Zuxin,Du, Hewei,Huang, Min,Du, Hewei,Zhang, Zuxin,Huang, Yiqin. 2016

[4]Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites. Wu, Huiling,Li, Jinjin,Dong, Dan,Liu, Ting,Zhang, Taotao,Zhang, Dianpeng,Liu, Weicheng.

[5]Effects of culture conditions on spore types of Clonostachys rosea 67-1 in submerged fermentation. Chen, Y. M.,Liu, J. F.,Li, S. D.,Ma, G. Z.. 2014

[6]Optimization of fermentation medium and conditions for mycelial growth and water-soluble exo-polysaccharides production by Isaria farinosa B05. Wang, P.,Jiang, X.,Jiang, Y.,Hu, X.,Jiang, Y.,Wang, P.,Hu, X.,Hwang, H..

[7]Overexpressing key component genes of the secretion pathway for enhanced secretion of an Aspergillus niger glucose oxidase in Trichoderma reesei. Wu, Yilan,Sun, Xianhua,Xue, Xianli,Luo, Huiying,Yao, Bin,Su, Xiaoyun,Wu, Yilan,Xie, Xiangming.

[8]Production and Characteristics of High Quality Vinegar from Sugarcane Juice. Lin, Bo,Li, Yang-Rui,Zheng, Feng-Jin,Sun, Jian,Li, Zhi-Chun. 2015

[9]A novel major facilitator transporter TrSTR1 is essential for pentose utilization and involved in xylanase induction in Trichoderma reesei. Huang, Zhen-Bang,Chen, Xiu-Zhen,Qin, Li-Na,Wu, Hong-Qing,Dong, Zhi-Yang,Huang, Zhen-Bang,Su, Xiao-Yun.

[10]Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01. Xia, Jin-lan,Nie, Zhen-yuan,Shan, Yang.

[11]Excellent waste biomass-degrading performance of Trichoderma asperellum T-1 during submerged fermentation. Wang, Qun,Shen, Qi,Zhao, Yuhua,Wang, Qun,Chen, Liang,Yu, Daobing,Lin, Hui. 2017

[12]Isolation and Identification of A Cellulolytic Bacterium from the Huguang Maar Lake. Cui, Lihong,Li, Jihua,Wei, Xiaoyi,Han, Donghui,Zhang, Yuliang. 2013

[13]Cellulase production by Trichoderma sp on apple pomace under solid state fermentation. Sun, Haiyan,Peng, Ming,Ge, Xiangyang,Hao, Zhikui. 2010

[14]Recyclable magnetic carboxymethyl chitosan/calcium alginate - cellulase bioconjugates for corn stalk hydrolysis. Jiang, Jianfang,Zhao, Jiaqi,He, Chunyang,Cui, Baodong,Xiong, Jun,Ao, Juan,Xiang, Guangyan,Jiang, Hao.

[15]Molecular cloning, expression, and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle, Apriona germari. Wei, Ya Dong,Lee, Kwang Sik,Gui, Zhong Zheng,Yoon, Hyung Joo,Kim, Iksoo,Zhang, Guo Zheng,Guo, Xijie,Sohn, Hung Dae,Jin, Byung Rae.

[16]N-linked glycosylation of a beetle (Apriona germari) cellulase Ag-EGase II is necessary for enzymatic activity. Wei, YD,Lee, KS,Gui, ZZ,Yoon, HJ,Kim, I,Je, YH,Lee, SM,Zhang, GZ,Guo, XJ,Sohn, HD,Jin, BR.

[17]Improvement of the catalytic performance of a Bispora antennata cellulase by replacing the N-terminal semi-barrel structure. Zheng, Fei,Wang, Xiaoyu,Xie, Xiangming,Zheng, Fei,Huang, Huoqing,Wang, Xiaoyu,Tu, Tao,Liu, Qiong,Meng, Kun,Wang, Yuan,Su, Xiaoyun,Luo, Huiying.

[18]Heterologous expression characteristics of trichoderma viride endoglucanase V in the Silkworm, Bombyx mori L.. Miao, Yun-gen,Li, Xing-hua,Wang, Mei-xian,Zhang, Peng,Hu, Jia-biao,Sun, Chun-guang,Zhou, Fang,Niu, Yan-shan,Malik, Firdose Ahmad,Bhaskar, Roy,Yang, Hua-jun,Miao, Yun-gen,Liu, Xin-ju.

[19]Fermentation quality and chemical composition of shrub silage treated with lactic acid bacteria inoculants and cellulase additives. Yu, Zhu,Zhao, Shufen,Cai, Yimin,Sun, Qizhong,Gao, Fengqin,Tao, Ya. 2012

[20]Insights into the roles of non-catalytic residues in the active site of a GH10 xylanase with activity on cellulose. Chu, Yindi,Tu, Tao,Xue, Xianli,Wang, Xiaoyu,Gong, Li,Luo, Huiying,Yao, Bin,Su, Xiaoyun,Penttinen, Leena,Rouvinen, Juha,Hakulinen, Nina,Yi, Zhuolin,Gong, Li. 2017

作者其他论文 更多>>

微信小程序