A highly-active endo-1,3-1,4-beta-glucanase from thermophilic Talaromyces emersonii CBS394.64 with application potential in the brewing and feed industries

文献类型： 外文期刊

第一作者： Wang, Kun

作者： Wang, Kun;Luo, Huiying;Shi, Pengjun;Huang, Huoqing;Bai, Yingguo;Yao, Bin

作者机构：

关键词： Talaromyces emersonii;Thermophilic;Feed and brewing industries

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2014 年 49 卷 9 期

页码：

收录情况： SCI

摘要： A 1245-bp endoglucanase gene of glycoside hydrolase (GH) family 7, egl7A, was cloned from the acidothermophilic fungus Talaromyces emersonii CBS394.64 and successfully expressed in Pichia pastoris. Sequence alignments indicated that Egl7A had highest identity of 62.7% at the amino acid level with the functionally characterized endoglucanase from Aspergillus terreus NIH2624. Purified recombinant Egl7A exhibited the maximum activity at pH 4.5 and 70 degrees C, retained stable over the pH range of 2.0-12.0 and at 65 degrees C, and was strongly resistant to acidic and neutral proteases, most metal ions and SOS. The enzyme exhibited the highest specific activity reported so far (11,299 U mg(-1)) when using barley beta-glucan as the substrate. Egl7A exhibited broad substrate specificity, including barley eta-glucan, lichenin, CMC-Na, and xylan and had capacity to cleave cellopentaose and cellohexaose into smaller units rapidly. Under simulated mashing conditions, addition of Egl7A reduced the mash viscosity by 12.40%; when combined with a GH10 xylanase, more viscosity reduction (27.75%) was observed, which is significantly higher than that of the commercial enzyme Ultraflo XL (17.91%). All these properties make Egl7A attractive for potential applications in the feed and brewing industries. (C) 2014 Elsevier Ltd. All rights reserved.

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