Cytotoxicity induced by ochratoxin A, zearalenone, and alpha-zearalenol: Effects of individual and combined treatment

文献类型: 外文期刊

第一作者: Wang, H. W.

作者: Wang, H. W.;Wang, J. Q.;Li, S. L.;Zhang, Y. D.;Zheng, N.;Wang, H. W.;Li, F. D.;Wang, H. W.;Wang, J. Q.;Li, S. L.;Zhang, Y. D.;Zheng, N.;Zheng, B. Q.

作者机构:

关键词: Ochratoxin A;Zearalenone;alpha-Zearalenol;Isobologram;Hep G2;Cytotoxicity

期刊名称:FOOD AND CHEMICAL TOXICOLOGY ( 影响因子:6.023; 五年影响因子:5.844 )

ISSN: 0278-6915

年卷期: 2014 年 71 卷

页码:

收录情况: SCI

摘要: This study investigated the cytotoxicity of combined mycotoxins of ochratoxin A (OTA), zearalenone (ZEA), anclior alpha-zearalenol (alpha-ZOL). The cytotoxicity of two mycotoxin combinations (two two-toxin combinations and one three-toxin combination) on human Hep G2 cells was evaluated using a tetrazolium salt (MTT) assay and isobologram analysis. Our results demonstrated significant cytotoxic effects of the two-toxin combination and the three-toxin combination on Hep G2 cells in a time- and concentration-dependent manner. The combination indexes (CI) were 2.73-7.67 for the OTA + ZEA combination and 1.23-17.82 for the OTA + alpha-ZOL combination after 24 h, 48 h, and 72 h of exposure at all inhibit concentration (IC) levels (IC10-IC90), indicating an antagonism. The CIs of the ZEA + alpha-ZOL combination were 1.29-2.55 after 24 h and 72 h of exposure (IC10-IC90), indicating an antagonism. The CIs of the ZEA + alpha-ZOL combination were 0.74-1.68 after 48 h of exposure, indicating synergism (IC80-IC90), additive effects (IC50-IC70), or antagonism (IC10-IC40). For the OTA + ZEA + alpha-ZOL combination, the CIs were 1.41-14.65 after 24 h, 48 h, and 72 h of exposure (IC10-IC90), indicating an antagonism. (C) 2014 Elsevier Ltd. All rights reserved.

分类号:

  • 相关文献

[1]Individual and combined cytotoxicity assessment of zearalenone with ochratoxin A or alpha-zearalenol by full factorial design. Zheng, N.,Gao, Y. N.,Wang, H. W.,Wang, J. Q.,Zheng, N.,Gao, Y. N.,Wang, J. Q.,Wang, H. W.,Liu, J.. 2018

[2]Evaluation of reduced toxicity of zearalenone as measured by the Hep G2 cell assay on degradation enzymes. He, Xingxing,Li, Yun,Gu, Wenshu,Sun, Yange,Sun, Xiulan,Li, Shuai,Li, Yun.

[3]Effects of aflatoxin B1 combined with ochratoxin A and/or zearalenone on metabolism, immune function, and antioxidant status in lactating dairy goats. Huang, Shuai,Fan, Caiyun,Wang, Shang,Cheng, Jianbo,Zheng, Nan,Jabar, Adil,Wang, Jiaqi,Cheng, Ming. 2018

[4]Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B-1, ochratoxin A and zearalenone in agro-food. Li, Xin,Li, Peiwu,Zhang, Qi,Li, Ran,Zhang, Wen,Zhang, Zhaowei,Ding, Xiaoxia,Tang, Xiaoqian,Li, Xin,Zhang, Qi,Li, Ran,Zhang, Zhaowei,Li, Xin,Li, Peiwu,Li, Peiwu,Zhang, Zhaowei,Ding, Xiaoxia,Li, Peiwu,Zhang, Qi,Li, Ran,Zhang, Wen,Tang, Xiaoqian. 2013

[5]Occurrence of mycotoxins in feed ingredients and complete feeds obtained from the Beijing region of China. Zhao, Lihong,Fan, Yu,Ma, Shanshan,Ji, Cheng,Ma, Qiugang,Zhang, Jianyun,Jia, Yaxiong,Sun, Lei.

[6]Occurrence of four mycotoxins in cereal and oil products in Yangtze Delta region of China and their food safety risks. Li, Rui,Wang, Xu,Zhou, Yu,Zhou, Ting,Yang, Dongxu,Wang, Qi. 2014

[7]Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal. Sun, Yaning,Xing, Guangxu,Yang, Jifei,Wang, Fangyu,Deng, Ruiguang,Zhang, Gaiping,Hu, Xiaofei,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[8]Simultaneously Testing Deoxynivalenol, T2 Toxin and Ochratoxin A in Wheat with a Colloidal Gold Test Strip and Matrix Solid-Phase Dispersion Cleaning. Jin, Nuo,Wang, Miao,Fan, Bei,He, Yan,Huang, Yatao,Wang, Fengzhong,Liu, Yang,Wang, Miao,Fan, Bei,He, Yan,Huang, Yatao,Wang, Fengzhong. 2017

[9]iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A. Xu, Wentao,Luo, Yunbo,Huang, Kunlun,Wang, Yan,Wang, Yan,Peng, Xiaoli,Yang, Zhuojun,Zhao, Weiwei,Xu, Wentao,Hao, Junran,Wu, Weihong,Shen, Xiao Li,Luo, Yunbo,Huang, Kunlun,Peng, Xiaoli,Shen, Xiao Li. 2017

[10]Advances in research of nephrotoxicity and toxic antagonism of ochratoxin A. Zhao, Tao,Shen, Xiao Li,Chen, Wenying,Liao, Xin,Yang, Jieyeqi,Zou, Yan,Wang, Yan,Fang, Cuilan. 2017

[11]Fate of ochratoxin A during wheat milling and some Chinese breakfast processing. Peng, Chunhong,Wang, Li,Li, Shurong,Peng, Chunhong,An, Fengping,Zhang, Le,Wang, Changgang,Peng, Chunhong,Wang, Yichun,Liu, Hongzhi.

[12]Novel fluoroimmunoassays for detecting ochratoxin A using CdTe quantum dots. Yao, Jingjing,Wang, Fangyu,Sun, Yaning,Xing, Guangxu,Deng, Ruiguang,Hu, Xiaofei,Zhang, Gaiping,Han, Junling,Zhang, Gaiping.

[13]Simultaneous Determination of Aflatoxins and Ochratoxin A in Bee Pollen by Low-Temperature Fat Precipitation and Immunoaffinity Column Cleanup Coupled with LC-MS/MS. Xue, XiaoFeng,Li, Yi,Selvaraj, Jonathan Nimal,Liu, Yang,Xue, XiaoFeng,Li, Yi,Zhao, Liuwei,Dong, Haimin,Liu, Fengmao. 2014

[14]In Vitro Glucuronidation of Ochratoxin A by Rat Liver Microsomes. Han, Zheng,Wu, Aibo,Han, Zheng,Tangni, Emmanuel K.,Callebaut, Alfons,Han, Zheng,Di Mavungu, Jose Diana,De Saeger, Sarah,Vanhaecke, Lynn. 2013

[15]Quantitative assessment of risk associated with dietary intake of mycotoxin ochratoxin A on the adult inhabitants in Shanghai city of PR China. Han, Zheng,Nie, Dongxia,Yang, Xianli,Wang, Jianhua,Wu, Aibo,Peng, Shaojie,Han, Zheng. 2013

[16]Ochratoxin A Producing Fungi, Biosynthetic Pathway and Regulatory Mechanisms. Wang, Yan,Wang, Liuqing,Liu, Fei,Wang, Qi,Selvaraj, Jonathan Nimal,Xing, Fuguo,Zhao, Yueju,Liu, Yang,Wang, Yan,Xing, Fuguo,Zhao, Yueju,Liu, Yang. 2016

[17]Modulation of Intestinal Epithelial Permeability in Differentiated Caco-2 Cells Exposed to Aflatoxin M1 and Ochratoxin A Individually or Collectively. Gao, Yanan,Li, Songli,Wang, Jiaqi,Luo, Chaochao,Zhao, Shengguo,Zheng, Nan,Gao, Yanan,Li, Songli,Wang, Jiaqi,Luo, Chaochao,Zhao, Shengguo,Zheng, Nan,Gao, Yanan,Li, Songli,Wang, Jiaqi,Luo, Chaochao,Zhao, Shengguo,Zheng, Nan. 2018

[18]A reliable and sensitive time-resolved fluorescent immunochromatographic assay (TRFICA) for ochratoxin A in agro-products. Majdinasab, Marjan,Li, Peiwu,Zhang, Qi,Li, Xin,Tang, Xiaoqian,Li, Jing,Majdinasab, Marjan,Sheikh-Zeinoddin, Mahmoud,Soleimanian-Zad, Sabihe,Li, Peiwu,Zhang, Qi,Li, Xin,Tang, Xiaoqian,Li, Jing,Li, Peiwu,Li, Peiwu.

[19]Changes in biosynthesis and metabolism of glutathione upon ochratoxin A stress in Arabidopsis thaliana. Wang, Yan,Zhao, Weiwei,Hao, Junran,Xu, Wentao,Luo, YunBo,Wu, Weihong,Huang, Kunlun,Xu, Wentao,Luo, YunBo,Yang, Zhuojun,Liang, Zhihong,Huang, Kunlun,Wang, Yan.

[20]Ultrasensitive and quantitative gold nanoparticle-based immunochromatographic assay for detection of ochratoxin A in agro-products. Majdinasab, Marjan,Li, Peiwu,Zhang, Qi,Li, Xin,Tang, Xiaoqian,Majdinasab, Marjan,Sheikh-Zeinoddin, Mahmoud,Soleimanian-Zad, Sabihe,Li, Peiwu,Zhang, Qi,Li, Xin,Tang, Xiaoqian,Li, Peiwu,Li, Peiwu.

作者其他论文 更多>>