Protection Evaluation of a New Attenuated ASFV by Deletion of the L60L and CD2v Genes against Homologous Challenge

文献类型： 外文期刊

第一作者： Yang, Jinjin

作者： Yang, Jinjin;Wang, Yu;Qian, Aidong;Yang, Jinjin;Zhu, Rongnian;Li, Nan;Zhang, Yanyan;Zhou, Xintao;Yue, Huixian;Li, Qixuan;Miao, Faming;Chen, Teng;Zhang, Fei;Zhang, Shoufeng;Hu, Rongliang;Yang, Jinjin;Zhu, Rongnian;Li, Nan;Zhang, Yanyan;Zhou, Xintao;Yue, Huixian;Li, Qixuan;Miao, Faming;Chen, Teng;Zhang, Fei;Zhang, Shoufeng;Hu, Rongliang

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关键词： African swine fever; African swine fever virus; recombinant virus; deletion; live attenuated vaccine candidate

期刊名称：VIRUSES-BASEL （ 影响因子：3.5； 五年影响因子：3.7 ）

ISSN：

年卷期： 2024 年 16 卷 9 期

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收录情况： SCI

摘要： African swine fever (ASF) is an acute infectious disease with a high mortality rate in both domestic and wild boars. Commercial vaccines or antiviral drugs for ASF were not available due to the complex diversity of the structure and genome of its pathogen African swine fever virus (ASFV). In recent years, there have been many reports on candidate strains of attenuated vaccines for ASFV. In this study, we obtained a recombinant virus named SY18 Delta L60L Delta CD2v by simultaneously deleting the L60L gene and CD2v gene from highly virulent strain SY18. In vitro, SY18 Delta L60L Delta CD2v displayed a decreased growth kinetic compared to that of parental SY18. In vivo, high doses (10(5) TCID50) of SY18 Delta L60L Delta CD2v can protect pigs (5/5) from attacks by the parental SY18 strain (10(2) TCID50). Low doses (10(2) TCID50) of SY18 Delta L60L Delta CD2v only protected 20% of pigs (1/5) from attacks by the parental SY18 strain (10(2) TCID50). The results indicated that the absence of these two genes in SY18 could induce protection against the homologous parental strain, and there were no obvious clinical symptoms or viremia. These results indicate that the SY18 Delta L60L Delta CD2v strain can serve as a new live attenuated vaccine candidate for the prevention and control of ASFV infection.

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