Extraction and purification of chlorogenic acid from ramie (Boehmeria nivea L. Gaud) leaf using an ethanol/salt aqueous two-phase system

文献类型： 外文期刊

第一作者： Tan, Zhijian

作者： Tan, Zhijian;Wang, Chaoyun;Yi, Yongjian;Wang, Hongying;Li, Mao;Zhou, Wanlai;Tan, Shiyong;Tan, Zhijian;Li, Fenfang

作者机构：

关键词： Ramie;Chlorogenic acid;Aqueous two-phase system;Purification

期刊名称：SEPARATION AND PURIFICATION TECHNOLOGY （ 影响因子：7.312； 五年影响因子：6.656 ）

ISSN： 1383-5866

年卷期： 2014 年 132 卷

页码：

收录情况： SCI

摘要： In this paper, an aqueous two-phase system (ATPS) based on ethanol/NaH2PO4 was used in separation and purification of chlorogenic acid (CGA) from ramie leaf. CGA was firstly extracted from dried ramie leaf powder by ethanol solution, then was added into ATPS for further purification. During the ATPS extraction procedure, the influencing parameters of type and concentration of salt, extraction temperature and pH were investigated. The experiments results demonstrated the CGA were extracted into alcohol-rich phase with impurities being extracted into salt-rich phase, the phase-forming components were recycled to reduce the cost after extraction. Furthermore, the thermodynamics and kinetics during the extraction procedure were simultaneously studied for the first time. This much easier, greener and cheaper alcohol/salt ATPS was more feasible for large-scale production. (C) 2014 Elsevier B.V. All rights reserved.

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