Adjuvant Effects of L. acidophilus LW1 on Immune Responses to the Foot- and-Mouth Disease Virus DNA Vaccine in Mice

文献类型： 外文期刊

第一作者： Su, Junhong

作者： Su, Junhong;Li, Jidong;Zheng, Haixue;You, Yanan;Luo, Xuenong;Li, Xueqiang;Ma, Xusheng;Li, Junjun;Dou, Yongxi;Cai, Xuepeng;Li, Jidong;Li, Yungang

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 8 期

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收录情况： SCI

摘要： The adjuvant effects of Lactobacillus acidophilus on DNA vaccination are not fully understood. It has been hypothesized that swine-derived Lactobacillus acidophilus SW1 (LASW1) could function as an immune adjuvant to enhance antigen-specific immune responses after foot-and-mouth disease (FMD) DNA vaccination in mice. To evaluate the effect of oral LASW1 on the immune response to a DNA vaccine (pRC/CMV-vp1) harboring FMD VP1 gene, anti-FMDV antibody and its isotypes, T-cell proliferation, and cytokine detection were investigated. The results showed that LASW1 was able to enhance FMDV-specific antibody levels and FMDV-neutralizing antibodies. After a booster vaccine, the anti-FMDV antibody titers and FMDV-neutralizing antibodies levels induced by pRC/CMV-vp1 were higher in mice treated with LSAW1 than in the group immunized with pRC/CMV-vp1 alone (the control). Using T-cell proliferation, the stimulation index of the LASW1 group was significantly higher in response to ConA and 146S antigen (P<0.05) than in the control group. Importantly, higher concentrations of IFN-gamma and IFN-gamma-producing cells were also observed in splenocytes isolated from the experimental LASW1 mice, indicating that INF-gamma secretion is important to the immune response to LASW1. The results indicate that LASW1 is a promising immune adjuvant in DNA vaccination against FMD when administrated orally.

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