Transcription of the Lysine-2,3-Aminomutase Gene in the kam Locus of Bacillus thuringiensis subsp kurstaki HD73 Is Controlled by Both sigma(54) and sigma(K) Factors

文献类型： 外文期刊

第一作者： Zhang, Zhe

作者： Zhang, Zhe;Yang, Min;Peng, Qi;Zhang, Jie;Song, Fuping;Wang, Guannan;Zheng, Qingyun

作者机构：

期刊名称：JOURNAL OF BACTERIOLOGY （ 影响因子：3.49； 五年影响因子：3.534 ）

ISSN： 0021-9193

年卷期： 2014 年 196 卷 16 期

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收录情况： SCI

摘要： Lysine 2,3-aminomutase (KAM; EC 5.4.3.2) catalyzes the interconversion of L-lysine and L-beta-lysine. The transcription and regulation of the kam locus, including lysine-2,3-aminomutase-encoding genes, in Bacillus thuringiensis were analyzed in this study. Reverse transcription-PCR (RT-PCR) analysis revealed that this locus forms two operons: yodT (yodT-yodS-yodR-yodQ-yodP-kamR) and kamA (kamA-yokU-yozE). The transcriptional start sites (TSSs) of the kamA gene were determined using 5' rapid amplification of cDNA ends (RACE). A typical -12/-24 sigma(54) binding site was identified in the promoter P-kamA, which is located upstream of the kamA gene TSS. A beta-galactosidase assay showed that P-kamA, which directs the transcription of the kamA operon, is controlled by the sigma(54) factor and is activated through the sigma(54)-dependent transcriptional regulator KamR. The kamA operon is also controlled by sigma(K) and regulated by the GerE protein in the late stage of sporulation. kamR and kamA mutants were prepared by homologous recombination to examine the role of the kam locus. The results showed that the sporulation rate in B. thuringiensis HD(Delta kamR) was slightly decreased compared to that in HD73, whereas that in HD(Delta kamA) was similar to that in HD73. This means that other genes regulated by KamR are important for sporulation.

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