Molecular Characterization of Atoxigenic Aspergillus flavus Isolates Collected in China
文献类型: 外文期刊
第一作者: Wei, Dandan
作者: Wei, Dandan;Zhou, Lu;Selvaraj, Jonathan Nimal;Zhang, Chushu;Xing, Fuguo;Zhao, Yueju;Wang, Yan;Liu, Yang
作者机构:
关键词: aflatoxins;Aspergillus flavus;atoxigenic isolate;deletion pattern;gene cluster;biological control
期刊名称:JOURNAL OF MICROBIOLOGY ( 影响因子:3.422; 五年影响因子:3.28 )
ISSN: 1225-8873
年卷期: 2014 年 52 卷 7 期
页码:
收录情况: SCI
摘要: Aspergillus flavus strains were isolated from peanut fields of Liaoning, Shandong, Hubei and Guangdong Provinces in China, and identified through phenotypic and molecular approaches. Of the 323 A. flavus strains isolated, 76 strains did not produce aflatoxins detectable by UPLC. The incidence of atoxigenic A. flavus strains decreased with increase in temperature and increased with increase in latitude in different geographical locations. Amplification of all the aflatoxin genes in the aflatoxin gene cluster in the atoxigenic isolates showed that there were 25 deletion patterns (A-Y), with 22 deletion patterns identified for the first time. Most of the atoxigenic A. flavus isolates with gene deletions (97%) had deletions in at least one of the four genes (aflT, nor-1, aflR, and hypB), indicating that these four genes could be targeted for rapid identification of atoxigenic strains. The atoxigenic isolates with gene deletions, especially the isolates with large deletions, are potential candidates for aflatoxin control.
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