Activity levels and expression of antioxidant enzymes in the ascorbate-glutathione cycle in artificially aged rice seed

文献类型： 外文期刊

第一作者： Yin, Guangkun

作者： Yin, Guangkun;Xin, Xia;Song, Chao;Chen, Xiaoling;Zhang, Jinmei;Wu, Shuhua;Li, Ruifang;Liu, Xu;Lu, Xinxiong

作者机构：

关键词： Artificial aging;Ascorbate-glutathione cycle;Rice seed

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2014 年 80 卷

页码：

收录情况： SCI

摘要： Reactive oxygen species are the main contributors to seed deterioration. In order to study scavenging systems for reactive oxygen species in aged seed, we performed analyses using western blotting, real-time quantitative reverse-transcription polymerase chain reaction, high-performance liquid chromatography, and antioxidant enzyme activity analyses in artificially aged rice seeds (Oryza sativa L cv. wanhua no.11). Aging seeds by storing them at 50 degrees C for 1, 9, or 17 months increased the superoxide radical and hydrogen peroxide levels and reduced the germination percentage from 99% to 92%, 55%, and 2%, respectively. The activity levels of superoxide dismutase (SOD), glutathione reductase (GR), and dehydroascorbate reductase (DHAR) did not change in aged seeds. In contrast, the activity levels of catalase (CAT), ascorbate peroxidase (APX), and monodehydroascorbate reductase (MDHAR) were significantly decreased in aged seeds, as were the expression of catalase and cytosolic ascorbate peroxidase protein. Transcript accumulation analysis showed that specific expression patterns were complex for each of the antioxidant enzyme types in the rice embryos. Overall, the expression of most genes was down-regulated, along with their protein expression. In addition, the reduction in the amount of ascorbate and glutathione was associated with the reduction in scavenging enzymes activity in aged rice embryos. Our data suggest that the depression of the antioxidant system, especially the reduction in the expression of CAT1, APX1 and MDHAR1, may be responsible for the accumulation of reactive oxygen species in artificially aged seed embryos, leading to a loss of seed vigor. (C) 2014 Elsevier Masson SAS. All rights reserved.

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