Construction and immunogenicity of a DNA vaccine coexpressing GP3 and GP5 of genotype-I porcine reproductive and respiratory syndrome virus

文献类型： 外文期刊

第一作者： Ren, Jing-Qiang

作者： Ren, Jing-Qiang;Sun, Wen-Chao;Lu, Hui-Jun;Wen, Shu-Bo;Jing, Jie;Yan, Fu-Long;Liu, Hao;Liu, Cun-Xia;Xiao, Peng-Peng;Chen, Xing;Du, Shou-Wen;Jin, Ning-Yi;Ren, Jing-Qiang;Jing, Jie;Xiao, Peng-Peng;Du, Shou-Wen;Jin, Ning-Yi;Sun, Wen-Chao;Wen, Shu-Bo;Yan, Fu-Long;Chen, Xing;Du, Rui;Ren, Jing-Qiang;Liu, Hao

作者机构：

关键词： Genotype-I PRRSV;GP3;GP5;DNA vaccine;Immunogenicity;Quil A

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2014 年 10 卷

页码：

收录情况： SCI

摘要： Background: The European (EU) genotype of porcine reproductive and respiratory syndrome virus (Genotype-I PRRSV) has recently emerged in China. The coexistence of Genotype-I and -II PRRSV strains could cause seriously affect PRRSV diagnosis and management. Current vaccines are not able to protect against PRRSV infection completely and have inherent drawbacks. Thus, genetically engineered vaccines, including DNA vaccine and live vector engineered vaccines, have been developed. This study aimed to determine the enhanced immune responses of mice inoculated with a DNA vaccine coexpressing GP3 and GP5 of a Genotype-I PRRSV. Results: To evaluate the immunogenicity of GP3 and GP5 proteins from European-type PRRSV, three DNA vaccines, pVAX1-EU-ORF3-ORF5, pVAX1-EU-ORF3 and pVAX1-EU-ORF5, were constructed, which were based on a Genotype-I LV strain (GenBank ID: M96262). BALB/c mice were immunized with the DNA vaccines; delivered in the form of chitosan-DNA nanoparticles. To increase the efficiency of the vaccine, Quil A (Quillaja) was used as an adjuvant. GP3 and GP5-specific antibodies, neutralizing antibodies and cytokines (IL-2, IL-4, IL-10 and IFN gamma) from the immunized mice sera, and other immune parameters, were examined, including T-cell proliferation responses and subgroups of spleen T-lymphocytes. The results showed that ORF3 and ORF5 proteins of Genotype-I PRRSV induced GP3 and GP5-specific antibodies that could neutralize the virus. The levels of Cytokines IL-2, IL-4, IL-10, and IFN-gamma. of the experimental groups were significantly higher than those of control groups after booster vaccination (P < 0.05). The production of CD3(+) CD4(+) and CD3(+) CD8(+) T lymphocyte was also induced. T lymphocyte proliferation assays showed that the PRRSV LV strain virus could stimulate the proliferation of T lymphocytes in mice in the experimental group. Conclusions: Using Quil A as adjuvant, Genotype-I PRRSV GP3 and GP5 proteins produced good immunogenicity and reactivity. More importantly, better PRRSV-specific neutralizing antibody titers and cell-mediated immune responses were observed in mice immunized with the DNA vaccine co-expressing GP3 and GP5 proteins than in mice immunized with a DNA vaccine expressing either protein singly. The results of this study demonstrated that co-immunization with GP3 and GP5 produced a better immune response in mice.

分类号：

  • 相关文献

[1]Enhanced immune responses in pigs by DNA vaccine coexpressing GP3 and GP5 of European type porcine reproductive and respiratory syndrome virus. Ren, Jingqiang,Jing, Jie,Xiao, Pengpeng,Jin, Ningyi,Ren, Jingqiang,Lu, Huijun,Wen, Shubo,Sun, Wenchao,Yan, Fulong,Chen, Xing,Jing, Jie,Liu, Hao,Liu, Cunxia,Xue, Fei,Xiao, Pengpeng,Xin, Shu,Jin, Ningyi,Wen, Shubo,Sun, Wenchao,Yan, Fulong,Chen, Xing,Xin, Shu,Liu, Hao.

[2]Evaluation of a DNA vaccine candidate co-expressing GP3 and GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) with interferon alpha/gamma in immediate and long-lasting protection against HP-PRRSV challenge. Qi, Jing,Wu, Jiaqiang,Liu, Xing,Zhang, Xiumei,Li, Jun,Sun, Wenbo,Cong, Xiaoyan,Shi, Jianli,Wang, Jinbao,Lu, Yu,Yoo, Dongwan. 2012

[3]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[4]Monoclonal antibodies and conserved antigenic epitopes in the C terminus of GP5 protein of the North American type porcine reproductive and respiratory syndrome virus. Zhou, Yan-Jun,Yu, Hai,Li, Guo-Xin,Jiang, Yi-Feng,Tong, Guang-Zhi,Tian, Zhi-Jun,Liu, Jin-Xia,An, Tong-Qing,Peng, Jin-Mei,Cai, Xue-Hui,Wang, Mei,Wang, Yun-Feng,Xue, Qiang.

[5]Molecular epidemiology of porcine reproductive and respiratory syndrome virus in Central China since 2014: The prevalence of NADC30-like PRRSVs. Wang, Lin-jian,Gu, Yu,Zhang, Gaiping,Wang, Lin-jian,Xie, Weitao,Chen, Xin-xin,Qiao, Songlin,Zhao, Mengmeng,Gu, Yu,Zhao, Bao-lei,Zhang, Gaiping,Zhao, Mengmeng,Zhao, Bao-lei,Zhang, Gaiping.

[6]Phylogenetic analysis and molecular characteristics of 17 porcine reproductive and respiratory syndrome virus isolates in Southern China from 2010 to 2011. Zhang, Minze,Xie, Jiexiong,Sun, Long,Cao, Zhenpeng,Gu, Honglang,Deng, Shengchao,Chen, Yao,Tang, Fengfeng,Su, Shuo,Zhang, Guihong,Cao, Zongxi.

[7]Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species. Tian, Lu,Li, Wenyu,Huang, Xinmei,Tian, Di,Liu, Jianhua,Yang, Xinchao,Liu, Lianrui,Yan, Ruofeng,Xu, Lixin,Li, Xiangrui,Song, Xiaokai,Huang, Xinmei. 2017

[8]Characterization and immunogenicity of an apxIA mutant of Actinobacillus pleuropneumoniae. Xu, Fuzhou,Chen, Xiaoling,Shi, Aihua,Yang, Bing,Wang, Jinluo,Li, Yongqing,Guo, Xin,Blackall, P. J.,Yang, Hanchun. 2006

[9]Biological Characteristics of Chinese Precocious Strain of Eimeria acervulina and Its Immune Efficacy Against Different Field Strains. Wu, Lin-Lin,Lin, Rui-Qing,Duan, Wen-Fang,Zou, Shang-Shu,Yuan, Zi-Guo,Weng, Ya-Biao,Sun, Ming-Fei,Liu, Li-Dan. 2014

[10]Identification and screening of effective protective antigens for channel catfish against Streptococcus iniae. Wang, Yajun,Wang, Erlong,He, Yang,Wang, Kaiyu,Yang, Qian,Wang, Jun,Geng, Yi,Ouyang, Ping,Lai, Weimin,Wang, Yajun,Wang, Kaiyu,Geng, Yi,Chen, Defang,Huang, Xiaoli. 2017

[11]Immunogenicity of the LrrG protein encapsulated in PLGA microparticles in Nile tilapia (Oreochromis niloticus) vaccinated against Streptococcus agalactiae. Ke, Xiaoli,Chen, Xue,Liu, Zhigang,Lu, Maixin,Gao, Fengying,Cao, Jianmeng,Chen, Xue. 2017

[12]Generation of a Recombinant Baculovirus Expressing The E2 Protein of Classical Swine Fever Virus and Its Immunogenicity in a Mouse Model. Miao, Li,Wang, Yu-Fei,Yu, Wang,Hui, Gao,Na, Li,Yuan, Sun,Liang, Bing-Bing,Qiu, Hua-Ji. 2009

[13]Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice. Li, Jun,Yu, Jiang,Xu, Shaojian,Shi, Jianli,Xu, Shengnan,Wu, Xiaoyan,Peng, Zhe,Zhang, Lingling,Zheng, Shuxuan,Yuan, Xiaoyuan,Cong, Xiaoyan,Sun, Wenbo,Cheng, Kaihui,Du, Yijun,Wu, Jiaqiang,Wang, Jinbao,Fu, Fang. 2016

[14]Enhancement of the Pharmacological Efficacy of FGF-21 by Genetic Modification and PEGylation. Ye, Xianlong,Qi, Jianying,Ren, Guiping,Zhu, Shenglong,Wu, Yunzhou,Yu, Dan,Liu, Mingyao,Li, Deshan,Sun, Guopeng,Zhao, Jingzhuang,Li, Deshan. 2013

[15]Immunogenicity of the glycoprotein 5 truncated trans-membrane regions of porcine reproductive and respiratory syndrome virus. Wang, Yinbiao,Xing, Guangxu,Zhang, Gaiping,Wang, Yinbiao,Xing, Guangxu,Guo, Junqing,Qiao, Songlin,Lu, Qingxia,Li, Qingmei,Zhang, Gaiping. 2011

[16]Development of a VLP-based vaccine in silkworm pupae against rabbit hemorrhagic disease virus. Zheng, Xuexing,Zheng, Xuexing,Wang, Shukun,Zhang, Weijiao,Liu, Xingjian,Yi, Yongzhu,Yang, Songtao,Xia, Xianzhu,Li, Yinv,Zhang, Zhifang,Zhang, Weijiao,Yang, Songtao,Xia, Xianzhu.

[17]Development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of S1 gene of H120 strain. Wei, Yan-Quan,Guo, Hui-Chen,Dong, Hu,Wang, Hai-Ming,Xu, Jin,Sun, De-Hui,Cai, Xue-Peng,Liu, Ding-Xiang,Sun, Shi-Qi,Liu, Ding-Xiang,Fang, Shou-Guo.

[18]Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice. Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua,Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua.

[19]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[20]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

作者其他论文 更多>>

微信小程序