Detection and sequence analysis of grapevine virus B isolates from China

文献类型： 外文期刊

第一作者： Hu, G. J.

作者： Hu, G. J.;Dong, Y. F.;Zhang, Z. P.;Fan, X. D.;Fang, R.;Zhu, H. J.

作者机构：

关键词： GVB;detection;sequence alignment;phylogeny

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN： 0001-723X

年卷期： 2014 年 58 卷 2 期

页码：

收录情况： SCI

摘要： The presence of grapevine virus B (GVB) was detected in 188 grapevine samples from China by double antibody sandwich ELISA (DAS-ELISA) and reverse transcription-PCR (RT-PCR). The accuracy of detection by RT-PCR was confirmed by sequencing amplified PCR fragments. Seventeen samples were GVB-positive by DAS-ELISA and five by RT-PCR. The isolate QMW proved to be positive by RT-PCR only, and four isolates (DGWH, DGW, QM, and JFL) could be detected by both methods. Among the five GVB-positive samples detected by RT-PCR, two isolates were originally collected from Henan province and three from Liaoning province. The expected 722 bp DNA fragment, covering partial ORF3 through partial ORF5, was amplified from the five GVB infected samples. Sequence analysis revealed that the molecular variants' composition of GVB in the different isolates was complex. Clones of DGWH, DGW, QM, and JFL isolate shared high nucleotide identities, while the identities among the clones of isolate QMW varied. The variants of GVB isolates obtained in this study showed nucleotide identities from 81.1% to 97.9% among themselves, and 79.1% to 98.5% identity with five previously published GVB isolates in NCBI. The alignment of partial ORF3 and the phylogenetic relationships of ORF4 revealed that the molecular variants of Chinese GVB isolates could be clustered into three groups. Only isolate DGW was in the same group with the reported GVB isolates from other countries; the other four GVB isolates in this study were clustered into two groups.

分类号：

  • 相关文献

[1]Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.). Li, Ai-Li,Zhu, Yuan-Fang,Tan, Xiao-Mei,Wang, Xiang,Wei, Bo,Guo, Han-Zi,Zhang, Zeng-Lin,Chen, Xiao-Bo,Zhao, Guang-Yao,Kong, Xiu-Ying,Jia, Ji-Zeng,Mao, Long,Tan, Xiao-Mei.

[2]A novel simple extracellular leucine-rich repeat (eLRR) domain protein from rice (OsLRR1) enters the endosomal pathway and interacts with the hypersensitive-induced reaction protein 1 (OsHIR1). Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhang, Qi,Lei, Cai-Lin,Zhang, Shi-Hong.

[3]Genome-wide analysis of SINA family in plants and their phylogenetic relationships. Wang, Meng,Jin, Ying,Fu, Junjie,Zhu, Yun,Hu, Jian,Wang, Guoying,Zheng, Jun,Wang, Guoying.

[4]Genome-wide analysis of AP2/ERF transcription factor family in Zea mays. Zhou, Mei-Liang,Tang, Yi-Xiong,Wu, Yan-Min,Zhou, Mei-Liang.

[5]Goose haemorrhagic hepatitis caused by a new subtype duck hepatitis type 1 virus. Liu, Ming,Fanyi-Meng,Li, Xiao-jun,Zhang, Zhuo,Liu, Siguo,Zhang, Yun.

[6]Molecular characterization and phylogenetic analysis of transmissible gastroenteritis virus HX strain isolated from China. Hu, Xiaoliang, Jr.,Li, Nannan, Jr.,Qu, Juanjuan,Hu, Xiaoliang, Jr.,Yin, Xin, Jr.,Tian, Zhige, Jr.,Qu, Liandong. 2015

[7]Fungal Biodiversity Profiles 21-30. Buyck, Bart,Duhem, Bernard,Hofstetter, Valerie,Das, Kanad,Parihar, Arvind,Jayawardena, Ruvishika S.,Niveiro, Nicolas,Michlig, Andrea,Fabian Popoff, Orlando,Andrea Ramirez, Natalia,Niveiro, Nicolas,Michlig, Andrea,Fabian Popoff, Orlando,Andrea Ramirez, Natalia,Lewish, David P.,Pereira, Olinto L.,da Silva, Meiriele,Prasher, Indu B.,Verma, Rajnish K.,Adhikari, Sinchan,Omar Alberto, Edgardo,Bulgakov, Timur S.,Castareda-Ruiz, Rafael F.,Hembrom, Manoj E.,Hyde, Kevin D.,Hyde, Kevin D.,Buyck, Bart,Nuytinck, Jorinde.

[8]Establishment and application of cross-priming isothermal amplification coupled with lateral flow dipstick (CPA-LFD) for rapid and specific detection of red-spotted grouper nervous necrosis virus. Su, Zi Dan,Shi, Cheng Yin,Huang, Jie,Shen, Gui Ming,Li, Jin,Wang, Sheng Qiang,Fan, Chao,Shi, Cheng Yin,Huang, Jie,Su, Zi Dan,Shen, Gui Ming,Wang, Sheng Qiang,Fan, Chao. 2015

[9]Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay. Liu, Tianqi,Yang, Bing,Song, Xiaoling,Wang, Xiuhua,Yuan, Yanyan,Liu, Li,Huang, Jie,Liu, Tianqi,Yuan, Yanyan. 2013

[10]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[11]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[12]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[13]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[14]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[15]Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR. Li, H.,Ni, D. H.,Li, J.,Li, H.,Ni, D. H.,Duan, Y. B.,Li, J.,Song, F. S.,Li, L.,Wei, P. C.,Yang, J. B.,Li, H.,Wei, P. C.,Duan, Y. B.,Song, F. S.,Chen, Y.. 2013

[16]Development of cross-priming amplification coupled with vertical flow visualization for rapid detection of infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish, Siniperca chuatsi. Liu, Wenzhi,Zhou, Yong,Fan, Yuding,Jiang, Nan,Zeng, Lingbing,Cain, Kenneth,Cain, Kenneth. 2018

[17]Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification. Liu, Yong-sheng,Liu, Xiang-tao. 2011

[18]The Applications of Gold Nanoparticle-Initialed Chemiluminescence in Biomedical Detection. Liu, Zezhong,Zhao, Furong,Gao, Shandian,Shao, Junjun,Chang, Huiyun. 2016

[19]A Miniature Handheld Automatic Detected Device for Register. Zhang Tiefeng,Wang Jun,Sun Zhihui,Zhao Bo,Lu Guotao. 2011

[20]DEVELOPMENT OF A SIMPLE AND EFFECTIVE METHOD FOR SPECIFIC DETECTION OF PEPPER MILD MOTTLE VIRUS. Liu, Y.,Wang, X.,Zhou, G.,Li, X.. 2009

作者其他论文 更多>>

微信小程序