A simple and rapid method for visualization of male meiotic chromosomes in Arabidopsis thaliana

文献类型: 外文期刊

第一作者: Li, Junhua

作者: Li, Junhua;Zhao, Xiting;Zhang, Xiaoli;Li, Mingjun;Yuan, Jinhong;Li, Junhua;Zhao, Xiting;Zhang, Xiaoli;Li, Mingjun

作者机构:

关键词: meiosis;chromosomes;cytogenetics;microscopy;Arabidopsis

期刊名称:BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY ( 影响因子:2.043; 五年影响因子:2.017 )

ISSN: 0916-8451

年卷期: 2014 年 78 卷 6 期

页码:

收录情况: SCI

摘要: Meiotic chromosomes are of basic interest to the geneticist and cell biologist who study their behavior. A rapid and highly repeatable method for visualization of meiotic chromosomes is useful. Here we describe a fast staining protocol for Arabidopsis male meiotic chromosomes. Meiocytes were squashed into a labeling buffer, the chromosome morphology could be analyzed using fluorescence without any additional treatment.

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