Purification and properties of heat-stable extracellular protease from Pseudomonads fluorescens BJ-10

文献类型： 外文期刊

第一作者： Zhang, Shuwen

作者： Zhang, Shuwen;Lv, Jiaping;Zhang, Shuwen;Lv, Jiaping

作者机构：

关键词： Pseudomonads fluorescens;Protease;Purification;Characterization;Milk;Spoilage

期刊名称：JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE （ 影响因子：2.701； 五年影响因子：3.574 ）

ISSN： 0022-1155

年卷期： 2014 年 51 卷 6 期

页码：

收录情况： SCI

摘要： Pseudomonas fluorescens BJ-10, a kind of psychrotrophic bacteria, was isolated from raw milk. It produced an extracellular protease of 47 kDa by SDS-PAGE. The crude proteases were purified by ammonium sulfate fractionation, ion-exchange and gel filtration chromatography. The specific activity of purified protease increased 61.38-fold. The optimum pH and temperature were pH 7.0 and 30 A degrees C, respectively. The purified protease was partially inhibited by DL-dithiothreitol, and the activity increased a little upon Fe2+ addition. The protease showed typical heat-stable behavior. After treatment at 100 A degrees C for 3 min, more than 94% activity remained. This work might lay the foundation for possible relationship between the heat stable protease and gelation of UHT milk.

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