Polydopamine-Functionalization of Graphene Oxide to Enable Dual Signal Amplification for Sensitive Surface Plasmon Resonance Imaging Detection of Biomarker

文献类型： 外文期刊

第一作者： Hu, Weihua

作者： Hu, Weihua;He, Guangli;Zhang, Huanhuan;Wu, Xiaoshuai;Li, Jialin;Zhao, Zhiliang;Qiao, Yan;Lu, Zhisong;Li, Chang Ming;Hu, Weihua;He, Guangli;Zhang, Huanhuan;Wu, Xiaoshuai;Li, Jialin;Zhao, Zhiliang;Qiao, Yan;Lu, Zhisong;Li, Chang Ming;Hu, Weihua;He, Guangli;Zhang, Huanhuan;Wu, Xiaoshuai;Li, Jialin;Zhao, Zhiliang;Qiao, Yan;Lu, Zhisong;Li, Chang Ming;Liu, Yang;Hu, Weihua

作者机构：

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）

ISSN： 0003-2700

年卷期： 2014 年 86 卷 9 期

页码：

收录情况： SCI

摘要： Surface plasmon resonance imaging (SPRi) is one of the powerful tools for immunoassays with advantages of label-free, real-time, and high-throughput; however, it often suffers from limited sensitivity. Herein we report a dual signal amplification strategy utilizing polydopamine (PDA) functionalization of reduced graphene oxide (PDA-rGO) nanosheets for sensitive SPRi immunoassay in serum. The PDA-rGO nanosheet is synthesized by oxidative polymerization of dopamine in a gentle alkaline solution in the presence of graphene oxide (GO) sheets and then is antibody-conjugated via a spontaneous reaction between the protein and the PDA component. In the dual amplification mode, the first signal comes from capture of the antibody-conjugated PDA-rGO to form sandwiched immunocomplexes on the SPRi chip, followed by a PDA-induced spontaneous gold reductive deposition on PDA-rGO to further enhance the SPRi signal. The detection limit as low as 500 pg mL(-1) is achieved on a nonfouling SPRi chip with high specificity and a wide dynamic range for a model biomarker, carcinoembryonic antigen (CEA) in 10% human serum.

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