Unveiling the development of cotton somatic embryos: Integrating spatial transcriptomics and metabolomics

文献类型： 外文期刊

第一作者： Qin, Aizhi

作者： Qin, Aizhi;Zhou, Yaping;Yu, Xiaole;Liu, Zhixin;Liu, Hao;Sun, Xuwu;Ge, Xiaoyang;Tran, Lam-Son Phan

作者机构：

关键词： Cotton; Spatial transcriptome; Spatial metabolome; Somatic embryo

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 230 卷

页码：

收录情况： SCI

摘要： Plant development necessitates a diverse array of cell types, metabolites, and intricate gene regulation patterns. Consequently, investigating the spatial localization of cells within tissues, the spatial expression of genes, and the distribution of both primary and secondary metabolites in plants is crucial. The process of somatic embryo development is vital for the genetic enhancement of cotton, yet it remains poorly understood. Moreover, there is currently a scarcity of available data resources on the spatial expression patterns of genes and metabolites in cotton somatic embryo cells. In this study, we employed 10 x Visium spatial transcriptomics (ST) technology and air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI) spatial metabolomics (SM) technology to establish sequencing libraries and metabolomes of upland cotton (Gossypium hirsutum) somatic embryos, specifically focusing on globular, torpedo, and cotyledonary embryos. Specifically, we have highlighted the spatially regulated gene networks and metabolite gradients critical to embryogenesis. For instance, the integrated analysis of ST and SM revealed that polyamines (putrescine, spermidine, and spermine) and their biosynthesis-related genes (ADC, ODC, ARGAH1, SPDSYN1, and SPMS) were highly expressed in globular embryos. These findings suggest that polyamines and their biosynthesis-related genes may play a pivotal role in the conversion of embryogenic callus into somatic embryos in cotton. In summary, we propose an integrated spatially resolved multiomics approach, which will aid in deciphering the complex regulatory networks involved in the development of cotton somatic embryos.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Genome-wide association study revealed candidate genes associated with leaf size in alfalfa (Medicago sativa L.)

    作者：Xu, Ming;Xu, Yanchao;Liu, Hao;Yang, Qingchuan;Long, Ruicai;Chen, Lin;He, Fei;Liu, Qingsong

    关键词：Alfalfa; GWAS; Leaf size; Haplotype analysis

  • Real-time visualization of "small Molecules-ssDNA" complexes for aptamer screening based on online competition CE-SELEX

    作者：Yang, Ge;Tian, Wenzhe;Hu, Yangyang;Wen, Yalun;Zhao, Yi;Qu, Feng;Yang, Ge;Jiang, Guangyu;Liu, Hao;Zhu, Chao;Zhao, Liping

    关键词：Aptamers; SELEX; CE-SELEX; Small molecules; Visualization

  • Resistance risk and mechanism of prochloraz in Fusarium solani

    作者：Mao, Xuewei;Cao, Tangbo;Chen, Liting;Li, Min;Zhao, Xingchen;Liu, Hao;Wang, Li;Zhou, Lin;Mao, Xuewei;Wang, Li;Zhou, Lin;Mao, Xuewei;Wang, Li;Zhou, Lin;Mao, Xuewei;Duan, Yun

    关键词：Fusarium solani; Prochloraz; Baseline sensitivity; Resistance mechanisms; Resistance management

  • Effects of Chicken Manure Combinations on Compost Maturity and Microbial Community Dynamics in Industrial-Scale Composting

    作者：Wang, Weiwei;Zhang, Peng;He, Huiban;Liu, Hao;He, Haoxing;Cui, Zongjun;Yuan, Xufeng;Yuan, Xufeng;Wen, Boting;Yan, Jing

    关键词：Aerobic composting; Chicken manure; Compost maturity; Microbial community

  • Climatic seasonality shapes insect community composition on the Mongolian Plateau

    作者：Tang, Kuanyan;Hilal, Mian Gul;Yue, Hongru;Ding, Xiaolong;Xing, Yifang;Zhao, Jinming;Liu, Yang;Lin, Kejian;Wang, Ning;Liu, Hao;He, Zilong

    关键词：Climatic seasonality; Steppe; Biodiversity; Insect community composition; PLS-PM model

  • Major Causative Bacteria of Dairy Cow Mastitis in the Inner Mongolia Autonomous Region, China, 2015-2024: An Epidemiologic Survey and Analysis

    作者：Zhao, Hongmei;Zhou, Yaping;Zhao, Fengmiao;Sun, Yajie;Wang, Yuchen;Bian, Yuchen;Tian, Guangyuan;Wu, Chunxia;Cui, Qi;Zhou, Xue;Hao, Yongqing;Guo, Ting;Cui, Jinlei;Si, Han

    关键词：cattle; mastitis; pathogens; environmental; contagious

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

微信小程序