Rapid detection of porcine kobuvirus in feces by reverse transcription loop-mediated isothermal amplification

文献类型： 外文期刊

第一作者： Li, Changlong

作者： Li, Changlong;Chen, Jianfei;Shi, Hongyan;Zhang, Xin;Shi, Da;Han, Xiao;Feng, Li;Chi, Yanbin

作者机构：

关键词： Porcine kobuvirus;Reverse transcription loop-mediated isothermal amplification (RT-LAMP);RT-PCR

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2014 年 11 卷

页码：

收录情况： SCI

摘要： Background: PKV is a new emerging pathogen detected in diarrhea pigs. At present, no more detection methods were reported except RT-PCR method. this study was to develop a fast diagnostic method based on the LAMP reaction for rapid detection of PKV nucleic acid in fecal samples. Findings: Two pairs of primers were designed to amplify the conservative 3D gene of PKV genome. The PKV RT-LAMP method possessed well specificity and had 100 times higher sensitivity than common reverse transcription PCR (RT-PCR), which could detect up to 10 RNA copies of the target gene. Conclusions: The results showed that the optimal reaction condition for RT-LAMP was achieved at 64 degrees C for 50 min. Furthermore, the RT-LAMP procedure does not demand special equipment and is time-saving.

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