文献类型: 外文期刊
第一作者: Xiong, Hui
作者: Xiong, Hui;He, Xiaohong;Zhang, Wenxiu;Li, Changli;Li, Mei;Guan, Weijun;Ma, Yuehui;Xiong, Hui
作者机构:
关键词: Duroc;fibroblasts;cell bank;genetic resources
期刊名称:PAKISTAN JOURNAL OF ZOOLOGY ( 影响因子:0.831; 五年影响因子:0.768 )
ISSN: 0030-9923
年卷期: 2014 年 46 卷 2 期
页码:
收录情况: SCI
摘要: A duroc fibroblast cell line has been established and the genetic resources have been long-term stored. We obtained the cell line by primary culture, subculture and cryopreservation, cells' viability were assessed, microbial or other organisms contamination were excluded, isoenzyme analysis and chromosome analysis were detected, three exogenous genes were transfect into the cells, results showed the freezing has little influence to the cell viability, the purity and the hereditary stability of the cell line were in good condition, which could provide abundant genetic materials for other researches in biological field.
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