Toxoplasma gondii: Protective immunity induced by rhoptry protein 9 (TgROP9) against acute toxoplasmosis

文献类型： 外文期刊

第一作者： Chen, Jia

作者： Chen, Jia;Zhou, Dong-Hui;Li, Zhong-Yuan;Huang, Si-Yang;Song, Hui-Qun;Zhu, Xing-Quan;Petersen, Eskild;Petersen, Eskild;Li, Zhong-Yuan;Zhu, Xing-Quan

作者机构：

关键词： Toxoplasma gondii;Toxoplasmosis DNA vaccine;Rhoptry protein 9 (ROP9);Protective immunity;Mice

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

ISSN： 0014-4894

年卷期： 2014 年 139 卷

页码：

收录情况： SCI

摘要： Toxoplasma gondii rhoptry protein 9 (ROP9) is involved in the early stages of host invasion, and contains B cell epitopes. The aim of this study was to evaluate the immune protective efficacy of a DNA vaccine encoding TgROP9 gene against acute T. gondii infection in mice. A DNA vaccine (pVAX-ROP9) encoding TgROP9 inserted into eukaryotic expression vector pVAX I was constructed, and the efficacy of intramuscular vaccination of Kunming mice with pVAX-ROP9 was analyzed. Mice immunized with pVAX-ROP9 induced a high level of specific anti-T. gondii antibodies, as well as a mixed IgG1/IgG2a response with predominance of IgG2a production. Also, injection of pVAX-ROP9 induced a specific lymphocyte proliferative responses and Th1-type cellular immune response with production of IFN-gamma and interleukin-2. The percentages of CD4+ and CD8+ T cells were significantly increased in mice immunized with pVAX-ROP9, compared to empty vector, PBS or blank controls. Immunization with pVAX-ROP9 significantly (P < 0.05) prolonged survival time (12.9 +/- 2.9 days) after challenge infection with the virulent T. gondii RH strain (Type I), compared with the control groups which died within 6 days. DNA vaccination with pVAX-ROP9 triggered strong humoral and cellular responses, and induced effective protection in mice against acute T. gondii infection, indicating that TgROP9 is a promising vaccine candidate against acute toxoplasmosis. (C) 2014 Elsevier Inc. All rights reserved.

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