Two xylose-tolerant GH43 bifunctional beta-xylosidase/alpha-arabinosidases and one GH11 xylanase from Humicola insolens and their synergy in the degradation of xylan
文献类型: 外文期刊
第一作者: Yang, Xinzhuo
作者: Yang, Xinzhuo;Shi, Pengjun;Huang, Huoqing;Luo, Huiying;Wang, Yaru;Yao, Bin;Zhang, Wei
作者机构:
关键词: Humicola insolens Y1;beta-Xylosidase;Xylanase;Xylose tolerance;Synergistic action
期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )
ISSN: 0308-8146
年卷期: 2014 年 148 卷
页码:
收录情况: SCI
摘要: Two beta-xylosidases of family 43 (Xyl43A and Xyl43B) and one xylanase of family 11 (Xyn11A) were identified from the genome sequence of Humicola insolens Y1, and their gene products were successfully expressed in heterologous hosts. The optimal activities of the purified Xyl43A. Xyl43B, and Xyn11 A were found at pH 6.5-7.0 and 50-60 degrees C. They were stable over a pH range of 5.0-10.0 and temperatures of 50 C and below. Xyl43A and Xyl43B had the activities of B-xylosidase, alpha-arabinosidase and xylanase, and showed xylose tolerance up to 79 and 292 mM, respectively. Xyn11A and Xyl43A or Xyl43B showed significant synergistic effects on the degradation of various xylans, releasing more reduced sugars (up to 1.29 folds) by simultaneous or sequential addition. This study provides several enzymes for synergistic degradation of xylan and contributes to the formulation of optimised enzyme mixtures for the efficient hydrolysis of plant biomass. (C) 2013 Elsevier Ltd. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Membralin is required for maize development and defines a branch of the endoplasmic reticulum-associated degradation pathway in plants
作者:Liu, Baiyu;Zhang, Ke;Qi, Shoumei;Jin, Zhe;Chen, Donghua;Zhang, Wei;Zhang, Kewei;Li, Kunpeng;Xu, Changzheng;He, Qiuxia;Cheng, Wen;Ding, Zhaohua;Zhao, Xiangyu
关键词:endoplasmic reticulum-associated degradation; maize; membralin; plant development; unfold protein response
-
Biocontrol performance and mass production potential of the larval endoparasitoid Campoletis chlorideae Uchida (Hymenoptera: Ichneumonidae) against the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
作者:Zhang, Rui;Zhang, Wei;Zhao, Qi;Keyhani, Nemat O.;Lei, Xian-Fu;Liu, Chang-Hua;Al Dhafer, Hathal M.;Mohamed, Amr
关键词:Spodoptera frugiperda; Field investigation; Campoletis chlorideae; Biological control; Mass production; Two-sex life table
-
Fluorescent Probes Based on Ag NPs@N/GQDs and Molecularly Imprinted Polymer for Sensitive Detection of Noradrenaline in Bananas
作者:Wang, Yaru;Wang, Mingyue;Liu, Sixin;Wang, Yaru;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Wu, Yuwei;Wang, Mingyue;Wang, Yaru;Li, Shuhuai;Wang, Mingyue;Li, Bei
关键词:Fluorescent probe; Graphene quantum dots; Silver nanoparticles; Molecular imprinted polymer; Noradrenaline
-
Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis
作者:Zhang, Hongmei;Zhang, Wei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Meng, Shan;Yan, Wei;Hui, Linchong;Chen, Wei
关键词:
-
Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis
作者:Sun, Chengming;Zhou, Xiaoying;Fu, Sanxiong;Wang, Xiaodong;Peng, Qi;Gao, Jianqin;Chen, Feng;Zhang, Wei;Hu, Maolong;Zhang, Jiefu;Wu, Jian;Liu, Huimin;Wang, Youping;Xue, Zhifei;Fu, Tingdong;Yi, Bin
关键词:Rapeseed; Branch angle; Homoeologous exchange; WGCNA; QTL-seq; WRKY40
-
A genome-wide association analysis for salt tolerance during the soybean germination stage and development of KASP markers
作者:Wang, Junyan;Zhou, Miaomiao;Su, Chengfu;Wang, Junyan;Zhou, Miaomiao;Zhang, Hongmei;Liu, Xiaoqing;Zhang, Wei;Wang, Qiong;Jia, Qianru;Chen, Huatao;Xu, Donghe;Chen, Huatao
关键词:soybean; salt tolerance; germination stage; genome-wide association analysis; KASP marker
-
Urea Coated with Polyaspartic Acid-Chitosan Increases Foxtail Millet (Setaria italica L. Beauv.) Grain Yield by Improving Nitrogen Metabolism
作者:Lu, Lin;Zhang, Wei;Xv, Yanli;Dong, Haosheng;Chen, Disu;Yan, Peng;Dong, Zhiqiang;Wang, Qi;Zhang, Wei;Dong, Zhiqiang;Gao, Ming;Li, Shujie
关键词:fertilizer; nitrate reductase; nitrogen availability; high yield and efficiency; one-time basic fertilizer application