文献类型: 外文期刊
第一作者: Zheng, Jun
作者: Zheng, Jun;Gong, Pengtao;Li, Mingying;Zhang, Guocai;Zhang, Xichen;Li, Jianhua;Zheng, Jun;Jia, Honglin
作者机构:
关键词: Eimeria tenella;Rhomboid;Microneme;Interaction
期刊名称:VETERINARY PARASITOLOGY ( 影响因子:2.738; 五年影响因子:2.951 )
ISSN: 0304-4017
年卷期: 2014 年 201 卷 1-2 期
页码:
收录情况: SCI
摘要: Invasion in several apicomplexan parasites, including Eimeria tenella, is accompanied by shedding of surface adhesins by intramembrane proteolysis mediated by rhomboid protease. We have previously identified E. tenella rhomboid 3 (EtROM3), but its precise role has not been elucidated. In this study, the interactions between EtROM3 and microneme (MIC) proteins were analyzed using the yeast two hybrid technique. The results showed that c-Myc-ROM3 fusion protein interacted with EtMIC4 protein in co-transformed AH109 yeasts, which was further confirmed by immunoprecipitation assay. Smaller EtMIC4 band from co-transformed cells suggested that EtROM3 was an active protease and involved in the cleavage of EtMIC4. (C) 2014 The Authors. Published by Elsevier B.V. All rights reserved.
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