Global characterization of microRNAs in Trichomonas gallinae

文献类型： 外文期刊

第一作者： Xu, Min-Jun

作者： Xu, Min-Jun;Qiu, Shen-Ben;Fu, Jing-Hua;Zhu, Xing-Quan;Nisbet, Alasdair J.;Qiu, Shen-Ben;Fu, Jing-Hua;Shao, Chang-Chun

作者机构：

关键词： Trichomonas gallinae;MicroRNA (miRNA);Quantitative RT-PCR;Gene regulation;Richomonosis

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2014 年 7 卷

页码：

收录情况： SCI

摘要： Background: Trichomonas gallinae is a protozoan parasite causing trichomonosis in many species of domestic poultry and birds world-wide. microRNAs (miRNAs) are a class of small non-coding RNAs that play key roles in gene regulation. However, no miRNAs have been characterized from T. gallinae. Methods: Here, we investigated the global miRNA profile of this parasite by high throughput sequencing technology, bioinformatics platform analysis and quantitative RT-PCR. Results: Three miRNA candidates, with typical precursor stem-loop structures, were identified from 11.13 million raw sequencing reads. Three miRNAs, Tga-miR-1, Tga-miR-2 and Tga-miR-3 had no homologues in publically available miRNA databases, suggesting that they may be T. gallinae-specific. Tga-miR-2 and Tga-miR-3 occupied only one location each on the reference genome, while Tga-miR-1 was found at 3 locations. Conclusions: The results of the present study provided a sound basis for the further understanding of gene regulation in this parasite of animal health significance, with the potential to inform the development of novel control reagents and strategies and also inform a more in-depth understanding of the evolution of miRNAs.

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