Identification and characterization of a novel endoglucanase (CMCase) isolated from the larval gut of Bombyx mori

文献类型: 外文期刊

第一作者: Ma, Ru-Jian

作者: Ma, Ru-Jian;Wang, Chun-Yan;Liu, Yan-Wei;Sivakumar, Thasma Raman;Ren, Zi-Xu;Fang, Ying;Jia, Jun-Qiang;Gui, Zhong-Zheng;Jia, Jun-Qiang;Gui, Zhong-Zheng

作者机构:

关键词: Bombyx mori;Cellulase;Identification;Enzymatic characteristics

期刊名称:JOURNAL OF ASIA-PACIFIC ENTOMOLOGY ( 影响因子:1.303; 五年影响因子:1.427 )

ISSN: 1226-8615

年卷期: 2014 年 17 卷 1 期

页码:

收录情况: SCI

摘要: While screening for cellulase-producing fungi from insect gut, a fungus with high endoglucanase (carboxymethyl cellulase; CMCase) activity was isolated from the larval gut of Bombyx mori. Based on morphological characteristics and using an 18S rRNA-based molecular phylogenetic approach, the fungus, strain BMC-2, was identified as a Mucor sp. expressing a novel alkalotolerant cellulase. The maximum production of cellulase by the BMC-2 strain was observed at 55 degrees C and pH 8.0. The CMCase activity was inhibited by Cu2+ > Na+ > Zn2+ > Mg2+ > Ba2+, and induced by Ca2+, Mn2+, Fe2+, and K+. Crown Copyright (C) 2013 Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. All rights reserved.

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