Generation of chimeric piglets by injection of embryonic germ cells from inbred Wuzhishan miniature pigs into blastocysts
文献类型: 外文期刊
第一作者: Dong, Xiao
作者: Dong, Xiao;Mu, Yulian;Liu, Lixin;Chen, Hongping;Zhang, Li;Feng, Shutang;Dong, Xiao;Tsung, Hsiaochien;Mu, Yulian;Feng, Shutang;Wang, Hongjun
作者机构:
关键词: chimera;embryonic germ cells;inbred miniature Wuzhishan pigs;primordial germ cells;xenotransplantation
期刊名称:XENOTRANSPLANTATION ( 影响因子:3.907; 五年影响因子:3.948 )
ISSN: 0908-665X
年卷期: 2014 年 21 卷 2 期
页码:
收录情况: SCI
摘要: Background Human embryonic stem/germ (ES/EG) cell research poses ethical dilemma, it is therefore critical to establish alternative sources of cells for relevant studies. Considering the similarities between the inbred miniature Wuzhishan pigs (WZSP) and humans, ES/EG from these pigs can serve as potential substitutes in human research. In this study, we reported our results that successfully established stable EG cell lines from the WZSP. Methods Primordial germ cells (PGCs) were isolated from the genital ridges of pig fetuses at 25 to 28 days of pregnancy. To obtain stable EG cell line, PGCs were maintained on STO cells in DMEM containing multiple essential growth factors. Results Two EG cell lines were established and characterized by positive alkaline phosphatase staining (AKP), expressions of Oct-4, SSEA-1, SSEA-3, SSEA-4, ability to differentiate into cells of all three germ layers in vitro, and generation of chimeric offsprings after microinjection and embryo transfer. Transmission electron microscopy demonstrated that the cytoplasmic structure of pig EG cells were rather simple and had a higher nuclear-to-cytoplasm ratio. Scanning electron microscopy showed the sizes of pig EG cells were similar to mouse EG cells. Both EG cell lines showed normal karyotypes. The EG cells were propagated for more than 20 passages and underwent multiple cycles of freezing and thawing, without losing their pluripotency (as distinguished by AKP staining). Conclusions Both in vitro and in vivo evidence strongly demonstrated that EG cells harvested from the inbred miniature WZSP were pluripotent and can be used for relevant pig or human studies.
分类号:
- 相关文献
作者其他论文 更多>>
-
Fusarium spp. Associated With Leaf Blight of Hosta (Hosta plantaginea L.) in China
作者:Jiao, Chenyang;Zhang, Li;Xu, Xi;Yang, Xilang;Guo, Lifeng;Wang, Xiangjing;Xiang, Wensheng;Zhao, Junwei;Guo, Lifeng;Xiang, Wensheng
关键词:
Fusarium spp.; Hosta; pathogenicity test; phylogenetic analysis -
Synergistic antimicrobial efficacy of glabrol and colistin through micelle-based co-delivery against multidrug-resistant bacterial pathogens
作者:Liu, Ying;Zhang, Mengying;Qu, Shaoqi;Liu, Ying;Mei, Chen;Wang, Hongjun;Cai, Yawei;Wu, Shuaicheng
关键词:Gram-negative bacteria; Colistin; Glabrol; Synergistic antibacterial; Micelle
-
TaFAR9 and TaFAR10 synergistically regulate fertility conversion of photo-thermo-sensitive genic male sterility lines in wheat by modulating ROS homeostasis
作者:Niu, Fuqiang;Geng, Qiang;Hu, Lingling;Zhang, Li;Gao, Xiaoran;Liu, Jinke;Song, Xiyue;Liu, Zihan;Bai, Jianfang;Liu, Yongjie;Yuan, Shaohua;Zhai, Nuo;Zhao, Changping;Zhang, Liping
关键词:Photo-thermo-sensitive genic male sterility; Fatty acyl CoA reductase; Fertility conversion; Reactive oxygen species; Wheat
-
The impact of nozzle spacing on the vortex evolution and dilution characteristics of pulsed jets in crossflow
作者:Wang, Chuan;Wang, Chuan;Yu, Hao;Zhang, Li;Wang, Chuan;Wang, Chuan;Li, Hao;Zhang, Li
关键词:Nozzle spacing; Pulse; Triple; Counter-rotating vortex pair; Diffusion
-
Identification of the bHLH Transcription Factor Family in Orah Mandarin and the Response of CrbHLH46 to Low-Temperature Stress
作者:Chen, Chaoying;Yin, Tuo;Tian, Ping;Zhao, Ke;Zhang, Hanyao;Li, Xulin;Wen, Ke;Liu, Xiaozhen;Zhang, Li;Zhou, Xianyan
关键词:Orah mandarin; bHLH transcription factor; transcriptome; low-temperature stress; expression pattern
-
Glutathione reductase modulates endogenous oxidative stress and affects growth and virulence in Avibacterium paragallinarum
作者:Zhi, Yan;Mei, Chen;Liu, Zhenyi;Liu, Ying;Wang, Hongjun
关键词:Glutathione reductase;
Av. paragallinarum ; Oxidative stress; Bacterial virulence; Redox homeostasis; Metabolic adaptation -
Haplotype-resolved genome assembly and genome-wide association study identifies the candidate gene closely related to sugar content and tuber yield in Solanum tuberosum
作者:Gong, Lei;Zhang, Guohui;Guo, Zhiqian;Zhang, Li;Zhang, Haiwen;Nie, Fengjie;Liu, Xuan;Yang, Wenjing;Zhang, Yu;Liu, Zhenning;Zhang, Hongxia;Fang, Miaoquan
关键词: