Multiplex PCR for diagnosis of Theileria uilenbergi, Theileria luwenshuni, and Theileria ovis in small ruminants

文献类型： 外文期刊

第一作者： Zhang, Xiao

作者： Zhang, Xiao;Liu, Zhijie;Yang, Jifei;Chen, Ze;Guan, Guiquan;Ren, Qiaoyun;Liu, Aihong;Luo, Jianxun;Yin, Hong;Li, Youquan;Zhang, Xiao;Liu, Zhijie;Yang, Jifei;Chen, Ze;Guan, Guiquan;Ren, Qiaoyun;Liu, Aihong;Luo, Jianxun;Yin, Hong;Li, Youquan;Zhang, Xiao;Liu, Zhijie;Yang, Jifei;Chen, Ze;Guan, Guiquan;Ren, Qiaoyun;Liu, Aihong;Luo, Jianxun;Yin, Hong;Li, Youquan

作者机构：

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2014 年 113 卷 2 期

页码：

收录情况： SCI

摘要： Infections with Theileria sp. may cause significant economic losses to the sheep industry. Species identification based on microscopic examination is difficult, and more suitable methods are required for the rapid detection and identification of Theileria sp, in clinical specimens. In this study, a multiplex polymerase chain reaction (mPCR) assay was developed to simultaneously identify three individual Theileria species in small ruminants. Three pairs of specific, sensitive primers were designed on the basis of the 5.8S ribosomal RNA gene (Theileria luwenshuni and Theileria ovis) and the 18S ribosomal RNA gene (Theileria uilenbergi) to generate target products of 303, 884, and 530 bp, respectively. Standard DNA for each of the three species was extracted from blood recovered from infected sheep, and a preliminary study was conducted on 56 sheep to verify the reliability of the system. Optimal PCR conditions, including primer concentration, annealing time, and the number of amplification cycles, were established. The assay sensitivity under these conditions was 10(-3) % parasitemia, and its specificity was 100 %. The results of the study suggest that mPCR represents a simple, efficient test method as a practical alternative for the rapid detection and identification of Theileria species in small ruminants.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序