The high-level accumulation of n-3 polyunsaturated fatty acids in transgenic pigs harboring the n-3 fatty acid desaturase gene from Caenorhabditis briggsae

文献类型： 外文期刊

第一作者： Zhou, Yanrong

作者： Zhou, Yanrong;Lin, Yanli;Wu, Xiaojie;Xiong, Fuyin;Chen, Hongxing;Feng, Chong;Long, Chuan;Wang, Ning;Pan, Dengke

作者机构：

关键词： n-3 fatty acid desaturase;cbr-fat-1;Transgenic pig;Somatic cell nuclear transfer

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN： 0962-8819

年卷期： 2014 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Livestock meat is generally low in n-3 polyunsaturated fatty acids (PUFAs), which are beneficial to human health. An alternative approach to increasing the levels of n-3 PUFAs in meat is to generate transgenic livestock animals. In this study, we describe the generation of cloned pigs that express the cbr-fat-1 gene from Caenorhabditis briggsae, encoding an n-3 fatty acid desaturase. Analysis of fatty acids demonstrated that the cbr-fat-1 transgenic pigs produced high levels of n-3 fatty acids from n-6 analogs; consequently, a significantly reduced ratio of n-6/n-3 fatty acids was observed. We demonstrated that the n-3 desaturase gene from C. briggsae was functionally expressed, and had a significant effect on the fatty acid composition of the transgenic pigs, which may allow the production of pork enriched in n-3 PUFAs.

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