Development of highly polymorphic simple sequence repeat markers using genome-wide microsatellite variant analysis in Foxtail millet [Setaria italica (L.) P. Beauv.]

文献类型： 外文期刊

第一作者： Zhang, Shuo

作者： Zhang, Shuo;Tang, Chanjuan;Li, Jing;Yang, Lifang;Qie, Lufeng;Fan, Xingke;Li, Lin;Zhao, Meicheng;Liu, Xiaotong;Chai, Yang;Zhang, Xue;Wang, Hailong;Li, Yingtao;Li, Wen;Zhi, Hui;Jia, Guanqing;Diao, Xianmin;Zhao, Qiang;Zhang, Ning;Li, Jing;Qie, Lufeng;Fan, Xingke;Li, Lin;Zhang, Xue;Wang, Hailong;Li, Yingtao;Diao, Xianmin;Li, Wen

作者机构：

关键词： Microsatellite marker;SSR development;Polymorphism;Setaria italica

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2014 年 15 卷

页码：

收录情况： SCI

摘要： Background: Foxtail millet (Setaria italica (L.) Beauv.) is an important gramineous grain-food and forage crop. It is grown worldwide for human and livestock consumption. Its small genome and diploid nature have led to foxtail millet fast becoming a novel model for investigating plant architecture, drought tolerance and C-4 photosynthesis of grain and bioenergy crops. Therefore, cost-effective, reliable and highly polymorphic molecular markers covering the entire genome are required for diversity, mapping and functional genomics studies in this model species. Result: A total of 5,020 highly repetitive microsatellite motifs were isolated from the released genome of the genotype 'Yugu1' by sequence scanning. Based on sequence comparison between S. italica and S. viridis, a set of 788 SSR primer pairs were designed. Of these primers, 733 produced reproducible amplicons and were polymorphic among 28 Setaria genotypes selected from diverse geographical locations. The number of alleles detected by these SSR markers ranged from 2 to 16, with an average polymorphism information content of 0.67. The result obtained by neighbor-joining cluster analysis of 28 Setaria genotypes, based on Nei's genetic distance of the SSR data, showed that these SSR markers are highly polymorphic and effective. Conclusions: A large set of highly polymorphic SSR markers were successfully and efficiently developed based on genomic sequence comparison between different genotypes of the genus Setaria. The large number of new SSR markers and their placement on the physical map represent a valuable resource for studying diversity, constructing genetic maps, functional gene mapping, QTL exploration and molecular breeding in foxtail millet and its closely related species.

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