Development and Application of an Immunoaffinity Column Enzyme Immunoassay for Mycotoxin Zearalenone in Complicated Samples
文献类型： 外文期刊
第一作者： Tang, Xiaoqian
作者： Tang, Xiaoqian;Li, Xin;Li, Peiwu;Zhang, Qi;Li, Ran;Zhang, Wen;Ding, Xiaoxia;Lei, Jiawen;Zhang, Zhaowei;Tang, Xiaoqian;Li, Xin;Li, Peiwu;Zhang, Qi;Li, Ran;Zhang, Wen;Lei, Jiawen;Zhang, Zhaowei;Tang, Xiaoqian;Li, Xin;Li, Peiwu;Zhang, Qi;Li, Ran;Zhang, Wen;Lei, Jiawen;Zhang, Zhaowei;Tang, Xiaoqian;Li, Xin;Li, Peiwu;Lei, Jiawen;Zhang, Zhaowei;Ding, Xiaoxia
作者机构：
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2014 年 9 卷 1 期
页码：
收录情况： SCI
摘要： The zearalenone (ZEA) monoclonal antibody (mAb) 2D3, one of the highest sensitivity antibodies, was developed. Based on this mAb, it was established of an immunoaffinity column (IAC) coupled with an indirect competitive enzyme-linked immunosorbent assay (icELISA). After optimization, the icELISA allowed an IC50 against ZEA of 0.02 mu g L-1. The mAb 2D3 exhibited a high recognition of ZEA (100%) and beta-zearalenol (beta-ZOL, 88.2%). Its cross-reactivity with a-zearalenol (alpha-ZOL) and beta-zearalanol (beta-ZAL) were found to be 4.4% and 4.6%, respectively. The IAC-icELISA method was employed to analyze ZEA contamination in food samples, compared with high-performance liquid chromatography (HPLC). The spiked assay for ZEA demonstrated the considerable recoveries for IAC-icELISA (83-93%) and HPLC (94-108%) methods. Results showed that the mAb 2D3 and IAC-icELISA method posed potential applications in sensitively determination of ZEA in maize.
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