Development and Application of an Immunoaffinity Column Enzyme Immunoassay for Mycotoxin Zearalenone in Complicated Samples
文献类型： 外文期刊
第一作者： Tang, Xiaoqian
作者： Tang, Xiaoqian;Li, Xin;Li, Peiwu;Zhang, Qi;Li, Ran;Zhang, Wen;Ding, Xiaoxia;Lei, Jiawen;Zhang, Zhaowei;Tang, Xiaoqian;Li, Xin;Li, Peiwu;Zhang, Qi;Li, Ran;Zhang, Wen;Lei, Jiawen;Zhang, Zhaowei;Tang, Xiaoqian;Li, Xin;Li, Peiwu;Zhang, Qi;Li, Ran;Zhang, Wen;Lei, Jiawen;Zhang, Zhaowei;Tang, Xiaoqian;Li, Xin;Li, Peiwu;Lei, Jiawen;Zhang, Zhaowei;Ding, Xiaoxia
作者机构：
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2014 年 9 卷 1 期
页码：
收录情况： SCI
摘要： The zearalenone (ZEA) monoclonal antibody (mAb) 2D3, one of the highest sensitivity antibodies, was developed. Based on this mAb, it was established of an immunoaffinity column (IAC) coupled with an indirect competitive enzyme-linked immunosorbent assay (icELISA). After optimization, the icELISA allowed an IC50 against ZEA of 0.02 mu g L-1. The mAb 2D3 exhibited a high recognition of ZEA (100%) and beta-zearalenol (beta-ZOL, 88.2%). Its cross-reactivity with a-zearalenol (alpha-ZOL) and beta-zearalanol (beta-ZAL) were found to be 4.4% and 4.6%, respectively. The IAC-icELISA method was employed to analyze ZEA contamination in food samples, compared with high-performance liquid chromatography (HPLC). The spiked assay for ZEA demonstrated the considerable recoveries for IAC-icELISA (83-93%) and HPLC (94-108%) methods. Results showed that the mAb 2D3 and IAC-icELISA method posed potential applications in sensitively determination of ZEA in maize.
分类号：
- 相关文献
作者其他论文 更多>>
-
Epigallocatechin-3-gallate-induced tolerance to cadmium stress involves increased flavonoid synthesis and nutrient homeostasis in tomato roots
作者：Wang, Yameng;Ahammed, Golam Jalal;Chen, Shuangchen;Wang, Yameng;Ge, Shibei;Shen, Keyin;Wang, Qianying;Wang, Wenli;Li, Xin;Gao, Haina
关键词：Heavy metal; Stress tolerance; Oxidative stress; Flavonoids; Nutrient homeostasis
-
Dual-template magnetic molecularly imprinted polymers for selective extraction and sensitive detection of aflatoxin B1 and benzo(α)pyrene in environmental water and edible oil
作者：Wang, Shenling;Hu, Xiaofeng;Wu, Wenqin;Wang, Du;Li, Peiwu;Zhang, Zhaowei;Zhang, Zhaowei
关键词：Dual dummy template; Surface molecularly imprinting; Magnetic solid phase extraction; Water sample
-
Silicon Improves the Plant Growth and Fruit Quality of Cherry Tomato (Solanum lycopersicum var. cerasiforme) under Nitrogen Imbalance by Modulating Nitrogen Assimilation and Photosynthesis
作者：Lei, Fei;Pan, Xiaozhong;Zhang, Zhijun;Zhang, Wen;Tan, Hao;Liu, Hailin;Lin, Huiru;Yang, Mei
关键词：Nitrogen; Silicon; Cherry tomato; Plant growth; Fruit quality
-
Moderate elimination of mycotoxins in vegetable oil triggered by superoxide anion and singlet oxygen
作者：Yang, Xianglong;Cheng, Ling;Yu, Li;Qi, Xin;Zhang, Liangxiao;Zhang, Qi;Mao, Jin;Li, Peiwu;Zhang, Liangxiao;Zhang, Qi;Mao, Jin;Li, Peiwu
关键词：Mycotoxins; Reactive oxygen species; Photodegradation; Bi2WO6
-
Fertilizer Effects on the Nitrogen Isotope Composition of Soil and Different Leaf Locations of Potted Camellia sinensis over a Growing Season
作者：Guo, Zuchuang;Huang, Tao;Guo, Zuchuang;Li, Chunlin;Shao, Shengzhi;Rogers, Karyne M.;Yuan, Yuwei;Li, Xin;Rogers, Karyne M.;Li, Qingsheng;Li, Da;Guo, Haowei
关键词：tea plants; Camellia sinensis; fertilizer; nitrogen isotopes; leaf locations; temporal variation
-
Species-specific IL-1β is an inflammatory sensor of Seneca Valley Virus 3C Protease
作者：Huang, Xiangyu;Zhao, Zhenchao;Chai, Lvye;Yan, Ya;Yuan, Ye;Wu, Lei;Li, Minjie;Jiang, Xiaohan;Li, Xin;Huang, Xiangyu;Zhao, Zhenchao;Chai, Lvye;Yan, Ya;Yuan, Ye;Wu, Lei;Li, Minjie;Jiang, Xiaohan;Li, Xin;Zhu, Cheng;Wang, Haiwei;Liu, Zheng;Li, Pingwei
关键词：
-
Microbiome resistance mediates stimulation of reduced graphene oxide to simultaneous abatement of 2,2′,4,4′,5-pentabromodiphenyl ether and 3,4-dichloroaniline in paddy soils
作者：Sun, Yi;Teng, Ying;Wang, Xia;Zhao, Ling;Sun, Yi;Teng, Ying;Wang, Xia;Zhao, Ling;Li, Ran
关键词：Microbiome resistance; Reduced graphene oxide; Polybrominated diphenyl ethers; Chloroanilines; Paddy soils
