Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus

文献类型： 外文期刊

第一作者： Xie, Chunliang

作者： Xie, Chunliang;Su, Haibo;Guo, Tianyao;Yan, Yizhong;Peng, Xiaozhen;Cao, Rui;Wang, Ying;Chen, Ping;Wang, Xianchun;Liang, Songping;Xie, Chunliang

作者机构：

关键词： Synaptotagmin I;Kv1.4;Interaction;Inactivation kinetics;Regulation

期刊名称：MOLECULAR BRAIN （ 影响因子：4.041； 五年影响因子：4.866 ）

ISSN： 1756-6606

年卷期： 2014 年 7 卷

页码：

收录情况： SCI

摘要： Background: The voltage-gated potassium channel Kv1.4 is an important A-type potassium channel and modulates the excitability of neurons in central nervous system. Analysis of the interaction between Kv1.4 and its interacting proteins is helpful to elucidate the function and mechanism of the channel. Results: In the present research, synaptotagmin I was for the first time demonstrated to be an interacting protein of Kv1.4 and its interaction with Kv1.4 channel did not require the mediation of other synaptic proteins. Using patch-clamp technique, synaptotagmin I was found to delay the inactivation of Kv1.4 in HEK293T cells in a Ca2+-dependent manner, and this interaction was proven to have specificity. Mutagenesis experiments indicated that synaptotagmin I interacted with the N-terminus of Kv1.4 and thus delayed its N-type fast inactivation. Conclusion: These data suggest that synaptotagmin I is an interacting protein of Kv1.4 channel and, as a negative modulator, may play an important role in regulating neuronal excitability and synaptic efficacy.

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