BcWRKY1 confers salt sensitivity via inhibiting Reactive oxygen species scavenging

文献类型： 外文期刊

第一作者： Yuan, Shuilin

作者： Yuan, Shuilin;Hu, Die;Wang, Yuan;Shao, Cen;Liu, Tongkun;Zhang, Changwei;Hou, Xilin;Li, Ying;Hu, Die;Cheng, Feng

作者机构：

关键词： BcWRKY1; BcZAT12; Salt stress; pak-choi; BcsAPX; ROS

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.335； 五年影响因子：5.197 ）

ISSN： 0167-4412

年卷期： 2022 年 109 卷 6 期

页码：

收录情况： SCI

摘要： WRKY transcription factors play important roles in abiotic stress by directly regulating stress-related genes. However, the molecular mechanism of its involvement in salt stress in pak-choi is still poorly understood. In this study, we elucidated the function of BcWRKY1 from pak-choi (Brassica rapa ssp. chinensis) in salt stress. The expression level of BcWRKY1 showed the highest in rosette leaves among different tissues and was induced by salt and ABA treatment in pak-choi. Subcellular localization showed that BcWRKY1 was located in nucleus. The transgenic Arabidopsis overexpressing BcWRKY1 exhibited enhanced salt sensitivity and higher H2O2 contents, which were further confirmed by silencing BcWRKY1 in pak-choi. In addition, the expression of ZAT12 was negatively regulated with BcWRKY1 under salt stress both in pak-choi and Arabidopsis. Yeast one-hybrid and dual luciferase reporter assay showed that BcWRKY1 could bind to the promoter of BcZAT12, and BcsAPX expression was activated by BcZAT12. To sum up, we propose a BcWRKY1-BcZAT12-BcsAPX regulatory model that involves in pak-choi salt stress response. Key message BcWRKY1 repress the expression of BcZAT12 to inhibit reactive oxygen species scavenging, leading to enhanced salt tolerance in BcWRKY1 silencing pak-choi.

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