Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine

文献类型： 外文期刊

第一作者： Sun, Tingting

作者： Sun, Tingting;Yu, Chao;Gao, Yuhua;Guan, Weijun;Ma, Yuehui;Sun, Tingting;Cai, Lianshun;Zhao, Chenqiong;Hua, Jinlian

作者机构：

关键词： bovine;cell culture;dermal mesenchymal stem cell (DMSC);multi-lineage differentiation

期刊名称：BIOSCIENCE REPORTS （ 影响因子：3.84； 五年影响因子：4.059 ）

ISSN： 0144-8463

年卷期： 2014 年 34 卷

页码：

收录情况： SCI

摘要： The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT-PCR (reverse transcription-PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.

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