Molecular Characterization of Enterohemorrhagic E-coli O157 Isolated from Animal Fecal and Food Samples in Eastern China

文献类型： 外文期刊

第一作者： Wang, Shaohui

作者： Wang, Shaohui;Zhang, Shuxiao;Liu, Zhe;Liu, Pingping;Shi, Zixue;Wei, Jianchao;Shao, Donghua;Li, Beibei;Ma, Zhiyong

作者机构：

期刊名称：SCIENTIFIC WORLD JOURNAL （ 影响因子：1.219； 五年影响因子：1.3 ）

ISSN： 1537-744X

年卷期： 2014 年

页码：

收录情况： SCI

摘要： Objective. To elucidate the extent of food contamination by enterohemorrhagic Escherichia coli (EHEC) O157 in Eastern China. Methods. A total of 1100 food and animal fecal samples were screened for EHEC O157. Then, molecular characterization of each isolate was determined. Results. EHEC O157 was isolated as follows: pig feces, 4% (20/500); cattle feces, 3.3% (2/60); chicken feces, 1.43% (2/140); pork, 2.14% (3/140), milk, 1.67% (1/60); and chicken meat, 1.67% (1/60). The stx1, stx2, eae, and hlyA genes were present in 26.7% (8/30), 40% (12/30), 63.3% (19/30), and 50% (15/30) of the O157 isolates, respectively. Molecular typing showed that strains from fecal and food samples were clustered into the same molecular typing group. Furthermore, the isolates from pork and pig feces possessed the same characterization as the clinical strains ATCC35150 and ATCC43889. Biofilm formation assays showed that 53.3% of the EHEC O157 isolates could produce biofilm. However, composite analyses showed that biofilm formation of EHEC O157 was independent of genetic background. Conclusions. Animal feces, especially from pigs, serve as reservoirs for food contamination by EHEC O157. Thus, it is important to control contamination by EHEC O157 on farms and in abattoirs to reduce the incidence of foodborne infections in humans.

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