Fine mapping and candidate gene analysis of qHD1b, a QTL that promotes flowering in common wild rice (Oryza rufipogon) by up-regulating Ehd1

文献类型： 外文期刊

第一作者： Liu, Ling

作者： Liu, Ling;Zhang, Yingxin;Yang, Qinqin;Zhang, Yue;Xu, Peng;Li, Jiaxin;Islam, Anowerul;Shah, Liaqat;Zhan, Xiaodeng;Cao, Liyong;Cheng, Shihua;Wu, Weixun;Liu, Ling;Zhang, Yingxin;Yang, Qinqin;Zhang, Yue;Xu, Peng;Li, Jiaxin;Islam, Anowerul;Shah, Liaqat;Zhan, Xiaodeng;Cao, Liyong;Cheng, Shihua;Wu, Weixun;Yang, Zhengfu;Cao, Liyong

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关键词： Rice; Heading date; Quantitative trait locus; qHD1b; Fine mapping

期刊名称：CROP JOURNAL （ 影响因子：4.647； 五年影响因子：5.781 ）

ISSN： 2095-5421

年卷期： 2022 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Heading date (flowering time) determines the adaptability of cultivars to different environments. We report the fine mapping and candidate gene analysis of qHD1b, a quantitative trait locus (QTL) responsible for early flowering that was derived from common wild rice (O. rufipogon) under both short-day and long-day conditions. The introgression line IL7391, which carried segments from common wild rice in a Zhonghui 8015 (ZH8015) background, exhibited early heading compared to the background and was crossed with ZH8015 to generate BC5F2:3 families for QTL analysis. This enabled the identification of two heading-date QTL, named qHD1b and qHD7, of which the first was selected for further research. High-resolution linkage analysis was performed in BC5F4:5 and BC5F6 populations, and the location of qHD1b was confined to a 112.7-kb interval containing 17 predicted genes. Five of these genes contained polymorphisms in the promoter or coding regions and were thus considered as candidates. Expression analysis revealed a positive association between LOC_Os01g11940 expression and early heading. This locus was annotated as OsFTL1, which encodes an ortholog of Arabidopsis Flowering Locus T and was the most likely candidate gene for qHD1b. Our study revealed that qHD1b acts as a floral activator that promotes flowering by up-regulating Ehd1, Hd3a, RFT1, OsMADS14, and OsMADS15 under both short -day and long-day conditions. Field experiments showed that qHD1b affected several yield-related agronomic traits including 1000-grain weight and grain length. qHD1b could be useful for marker-assisted selection and breeding of early-maturing cultivars. (C) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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