Mutational analysis of the Verticillium dahliae protein elicitor PevD1 identifies distinctive regions responsible for hypersensitive response and systemic acquired resistance in tobacco

文献类型： 外文期刊

第一作者： Liu, Zhipeng

作者： Liu, Zhipeng;Liu, Wenxian;Zeng, Hongmei;Yang, Xiufen;Guo, Lihua;Qiu, Dewen

作者机构：

关键词： Elicitor;PevD1;Hypersensitive response;Systemic acquired resistance;Verticillium dahliae

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）

ISSN： 0944-5013

年卷期： 2014 年 169 卷 5-6 期

页码：

收录情况： SCI

摘要： In our previous study, PevD1 was characterized as a novel protein elicitor produced by Verticillium dahliae inducing hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco plants; however, the detailed mechanisms of PevD1's elicitor activity remain unclear. In this study, five mutant fragments of PevD1 were generated by polymerase chain reaction-based mutagenesis and the truncated proteins expressed in Escherichia coli were used to test their elicitor activities. Biological activity analysis showed that the N-terminal and C-terminal of PevD1 had distinct influence on HR and SAR elicitation. Fragment PevD1 Delta N98, which spans the C-terminal 57 amino acids of PevD1, was critical for the induction of HR in tobacco plants. In contrast, fragment PevD1 Delta C57, the N-terminal of 98 amino acids of PevD1, retained the ability to induce SAR against tobacco mosaic virus (TMV) but not induction of HR, suggesting that the induction of HR is not essential for SAR mediated by PevD1. Our results indicated that fragment PevD1 Delta C57 could be a candidate peptide for plant protection against pathogens without causing negative effects. (C) 2013 Elsevier GmbH. All rights reserved.

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