Simultaneous Determination of Matrine and Berberine in Fruits, Vegetables, and Soil Using Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry

文献类型： 外文期刊

第一作者： Tian, Yingying

作者： Tian, Yingying;Dong, Fengshou;Xu, Jun;Li, Yuanbo;Liang, Xuyang;Zheng, Yongquan;Wang, Yunhao

作者机构：

期刊名称：JOURNAL OF AOAC INTERNATIONAL （ 影响因子：1.913； 五年影响因子：1.754 ）

ISSN： 1060-3271

年卷期： 2014 年 97 卷 1 期

页码：

收录情况： SCI

摘要： An ultra-performance LC/tandem MS (UPLC/MS/MS) method for the simultaneous quantification and identification of matrine and berberine, alkaloids widely used in plant fungicides, has been developed and validated. Methanol or 1% ammonia acetonitrile were selected as extraction solvents, and primary secondary amine sorbent was chosen for cleanup to achieve satisfactory recoveries in accordance with European Union guidelines. The chromatographic separation was carried out on a hydrophilic interaction LC column with a UPLC/MS/MS-based method to improve the retentions and shapes of the peaks. Method validation was performed for linearity, repeatability, interday precision, and sensitivity. Recoveries and RSDs were acceptable (73.1-109.3% recovery, RSD <= 15.8%). The LODs varied from 0.34 to 1.07 mu g/kg for matrine and 0.09 to 0.18 mu g/kg for berberine, while the LOQs ranged from 1.12 to 3.58 mu g/kg for matrine and 0.31 to 0.60 mu g/kg for berberine. This method was successfully and efficiently applied for the analysis of matrine and berberine in real samples.

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