Characterization of four midgut aminopeptidase N isozymes from Ostrinia furnacalis strains with different susceptibilities to Bacillus thuringiensis

文献类型: 外文期刊

第一作者: Wang, Zhenying

作者: Wang, Zhenying;Zhang, Jie;He, Kanglai;Xu, Lina;Ferry, Natalie;Edwards, Martin G.;Gatehouse, Angharad M. R.;Xu, Lina;Ferry, Natalie

作者机构:

关键词: Aminopeptidase N;cDNA;mRNA expression;Bt receptors;Ostrinia furnacalis

期刊名称:JOURNAL OF INVERTEBRATE PATHOLOGY ( 影响因子:2.841; 五年影响因子:3.368 )

ISSN: 0022-2011

年卷期: 2014 年 115 卷

页码:

收录情况: SCI

摘要: The full-length cDNA of four Ofapn aminopeptidases were cloned and sequenced from susceptible and resistant Ostrinia furnacalis strains. Four sequences were identified as APN because they shared the common structural features with APN from Lepidoptera, including the signal peptide, GPI anchor signal, the zinc binding/gluzincin motif HEX2HX18E and the gluzincin aminopeptidase motif GAMEN. Compared with APN sequences from the susceptible strain, there were 9, 5, 10 and 12 amino acid variations in the deduced protein sequences from the resistant strain. There were also differences in mRNA expression of the four Ofapn genes between resistant and susceptible O. furnacalis strains. (C) 2013 Published by Elsevier Inc.

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