Identification and characterization of (E)-beta-caryophyllene synthase and alpha/beta-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton
文献类型: 外文期刊
第一作者: Xiao, Yutao
作者: Xiao, Yutao;Zhang, Wanna;Wu, Juan;Guo, Yuyuan;Zhang, Yongjun;Huang, Xinzheng;Wu, Junxiang;Koellner, Tobias G.
作者机构:
关键词: alpha-Pinene;beta-Caryophyllene;Quantitative real-time PCR;Apolygus lucorum;Methyl jasmonate;Plant defense
期刊名称:PLANT PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:4.27; 五年影响因子:4.816 )
ISSN: 0981-9428
年卷期: 2013 年 73 卷
页码:
收录情况: SCI
摘要: Cotton (Gossypium hirsutum L) plants damaged by insects emit a blend of volatiles, including mono-terpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. To understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, GhTPS1 and GhTPS2, were heterologously expressed and characterized. Recombinant GhTPS1 accepted famesyl pyrophosphate as substrate and produced (E)-beta-caryophyllene and alpha-humulene. GhTPS2 was characterized as a monoterpene synthase which formed alpha-pinene and beta-pinene using geranyl pyrophosphate as substrate. Quantitative real-time PCR analysis revealed that GhTPS1 and GhTPS2 gene expression was elevated after methyl jasmonate (MeJA) treatment in cotton leaves. Moreover, feeding of the green plant bug Apolygus lucorum, a major cotton pest in northern China, resulted in increased GhTPS2 expression in young leaves, suggesting that GhTPS2 might be involved in plant defense in cotton. (C) 2013 Elsevier Masson SAS. All rights reserved.
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