Antigenic Analysis of Monoclonal Antibodies against Different Epitopes of sigma B Protein of Avian Reovirus

文献类型： 外文期刊

第一作者： Yin, Chun-hong

作者： Yin, Chun-hong;Qin, Li-ting;Sun, Mei-yu;Gao, Yu-long;Qi, Xiao-le;Gao, Hong-lei;Wang, Yong-qiang;Wang, Xiao-mei

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期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2013 年 8 卷 11 期

页码：

收录情况： SCI

摘要： Background: Avian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. GB is one of the major structural proteins of ARV, which is able to induce group-specific antibodies against the virus. Methods and Results: The present study described the identification of two linear B-cell epitopes in ARV sigma B through expressing a set of partially overlapping and consecutive truncated peptides spanning sigma B screened with two monoclonal antibodies (mAbs) 1F4 and 1H3-1. The data indicated that (21)KTPACW(26) (epitope A) and (WDTVTFH38)-W-32 (epitope B) were minimal determinants of the linear B cell epitopes. Antibodies present in the serum of ARV-positive chickens recognized the minimal linear epitopes in Western blot analyses. By sequence alignment analysis, we determined that the epitopes A and B were not conserved among ARV, duck reovirus (DRV) and turkey reovirus (TRV) strains. Western blot assays, confirmed that epitopes A and B were ARV-specific epitopes, and they could not react with the corresponding peptides of DRV and TRV. Conclusions and Significance: We identified (21)KTPACW(26) and (WDTVTFH38)-W-32 as sigma B -specific epitopes recognized by mAbs 1F4 and 1H3-1, respectively. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against ARV groups.

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