Nonstructural protein P7-2 encoded by Rice black-streaked dwarf virus interacts with SKP1, a core subunit of SCF ubiquitin ligase

文献类型： 外文期刊

第一作者： Wang, Qian

作者： Wang, Qian;Wang, Qian;Tao, Tao;Han, Yanhong;Chen, Xiangru;Fan, Zaifeng;Li, Dawei;Yu, Jialin;Han, Chenggui;Wang, Qian;Tao, Tao;Han, Yanhong;Chen, Xiangru;Fan, Zaifeng;Li, Dawei;Yu, Jialin;Han, Chenggui

作者机构：

关键词： Rice black-streaked dwarf virus;P7-2;SKP1;Interaction;SCF ubiquitin ligase

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2013 年 10 卷

页码：

收录情况： SCI

摘要： Background: Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus within the family Reoviridae, causes severe damage to cereal crops in South East Asia. The protein P7-2, encoded by the second open reading frame of segment S7, is conserved among most plant-infecting fijiviruses, but its function is still obscure. Results: In this study, P7-2 was used as bait in two-hybrid screens of a cDNA library expressing Zea mays proteins. It was found that there is a strong interaction between P7-2 and Z. mays SKP1 (SKP1(Maize)), a core subunit of the multicomponent SCF (SKP1/Cullin1/F-box/Rbx1) E3 ubiquitin ligase. The interaction was then confirmed in leaf epidermal cells of Nicotiana benthamiana by bimolecular fluorescence complementation assay. Further investigations indicated that P7-2 also interacts with SKP1 proteins from other plants, including Arabidopsis thaliana, N. benthamiana, Oryza sativa and Saccharum sinense. The C-terminal fragment of SKP1(Maize) (residues 97-176) and the middle fragment of P7-2 (residues 79-214) are necessary to sustain the interaction, while the C-terminal putative alpha-helix domain spanning residues 214-295 of P7-2 greatly facilitates the interaction. Agrobacterium-mediated transient suppression assay showed that P7-2 has no obvious activity to suppress local RNA silencing. Conclusions: Taken together, our results indicated that RBSDV P7-2 can interact with SKP1 proteins from different plants. This is the first report linking a Fijivirus protein to a component of the ubiquitin proteasome system. P7-2 might be a potential F-box protein encoded by RBSDV and involved in the plant-virus interaction through ubiquitination pathway.

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