Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp pekinensis)

文献类型： 外文期刊

第一作者： Xu, Xiaoyong

作者： Xu, Xiaoyong;Sun, Xilu;Zhang, Jing;Huang, Weiwei;Zhang, Lugang;Xu, Xiaoyong;Sun, Xilu;Zhang, Jing;Zhang, Lugang;Fang, Zhiyuan;Huang, Weiwei;Fang, Zhiyuan

作者机构：

关键词： cDNA-AFLP;CMS;Heading Chinese cabbage;qRT-PCR;Candidate gene

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN： 0137-5881

年卷期： 2013 年 35 卷 11 期

页码：

收录情况： SCI

摘要： Polima (pol) cytoplasmic male sterility (CMS) is reported to be regulated by orf224, but the molecular mechanisms of CMS are less known. In previous research, CMS7311 of heading Chinese cabbage (Brassica campestris L.ssp. pekinensis) was successfully generated from a pol-like CMS, named 77A. To further understand the molecular mechanisms of this male sterility, cDNA-AFLP and qRT-PCR were used to identify genes differentially expressed during flower bud development between cytoplasmic male-sterile plant (Ms) and male-fertile plant (Mf) derived from the hybrid of CMS7311 and a restorer (Rf). Thirty-two transcripts of different fragments (TDFs) of over 80 bp in length were identified using cDNA-AFLP. Among them, ten TDFs were found expressing in Ms bud, and the others expressing in Mf bud. Gene ontology analysis revealed that these genes were involved in stress response, synthesis and metabolism of fatty acid, cell reconstruction, etc. Further, five genes closely related to male sterile were selected and their expression during the flower bud development of Ms and Mf line were investigated by qRT-PCR. Three genes, BcGRP17, BcMS2, and BcPME31, which were necessary for normal male organ development, showed significantly lower expressions in Ms bud; two genes, BcROPGEF8 and BcTNL3, were induced with significantly higher expression in Ms bud by unknown stress and involved in the formation of male sterility. Further work will be focused on cloning these genes and identifying their functions in the male-sterile process of heading Chinese cabbage CMS7311.

分类号：

  • 相关文献

[1]Identification of SRAP markers linked to the restorer gene of CMS7311 in heading Chinese cabbage [Brassica rapa L. subsp pekinensis (Lour.) Olsson]. Xu, X.,Zhang, Y.,Hui, M.,Zhang, M.,Zhang, L.,Xu, X.,Zhang, Y.,Hui, M.,Zhang, M.,Zhang, L.,Fang, Z.. 2016

[2]Identification of sequence-related amplified polymorphism and insertion-deletion markers linked to the male fertility restorer gene of pol-like CMS06J45 in heading Chinese cabbage (Brassica rapa subsp pekinensis). Xu, X. Y.,Zhang, Y.,Zhang, L. G.,Xu, X. Y.,Fang, Z. Y.. 2014

[3]Overexpression of a heading Chinese cabbage ICE1 gene confers freezing tolerance in transgenic rice. Xiang, Dianjun,Man, Lili,Chai, Yongshan,Sun, Yuyou,Zhang, Taizhong,Wei, Caiqiang,Xie, Zhong,Li, Hongliang,Zhang, Weiwei,Liu, Dan,Cheng, Dujuan,Wang, Xiaodong,Liu, Chunguang.

[4]Linkage analysis of a fertility restoring mutant generated from CMS rice. Shen, YW,Guan, ZQ,Lu, J,Zhuang, JY,Zheng, KL,Gao, MW,Wang, XM. 1998

[5]Physical mapping and candidate gene prediction of fertility restorer gene of cytoplasmic male sterility in cotton. Zhao, Cunpeng,Zhao, Guiyuan,Geng, Zhao,Wang, Zhaoxiao,Wang, Kaihui,Liu, Suen,Zhang, Hanshuang,Guo, Baosheng,Geng, Junyi. 2018

[6]Somatic cybridization between Nicotiana tabacum and N-repanda based on a single inactivation procedure of nuclear donor parental protoplasts. Sun, YH,Xue, QZ,Ding, CM,Zhang, XY,Zhang, LL,Wang, WF,Ali, S.

[7]Effects of male sterile cytoplasm on yield and agronomic characters in Japonica hybrid rice, Oryza sativa L.. Wang, CL,Tang, SZ,Tang, YG. 1998

[8]A comparative light and electron microscopic analysis of microspore and tapetum development in fertile and cytoplasmic male sterile radish. Shi, Shengli,Ding, Ding,Wang, Jianbo,Mei, Shiyong.

[9]Analysis of Differentially Expressed Genes in Genic Male Sterility Cotton (Gossypium hirsutum L.) Using cDNA-AFLP. Xiaoding Ma,Chaozhu Xing,Liping Guo,Yangcang Gong,Hailin Wang,Yunlei Zhao,Jianyong Wu. 2007

[10]The Differential Expression of Resistant Genes Related to Cucumber Scab Displayed by cDNA-AFLP. Sun Xun,Li Xixiang,Qiu Yang,Shen Di,Song Jiangping,Sun Xun,Ren Ruixing. 2010

[11]Tomato defense to the powdery mildew fungus: differences in expression of genes in susceptible, monogenic- and polygenic resistance responses are mainly in timing. Li, Chengwei,Bai, Yuling,Jacobsen, Evert,Visser, Richard,Lindhout, Pim,Bonnema, Guusje. 2006

[12]A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans. Guo, J,Jiang, RHY,Kamphuis, LG,Govers, F. 2006

[13]Diversity and Genetic Differentiation of the Whitefly Bemisia tabaci Species Complex in China Based on mtCOI and cDNA-AFLP Analysis. Guo Xiao-jun,Rao Qiong,Zhang Fan,Luo Chen,Guo Xiao-jun,Gao Xi-wu,Rao Qiong,Zhang Hong-yu. 2012

[14]Comparative expression analysis in susceptible and resistant Gossypium hirsutum responding to Verticillium dahliae infection by cDNA-AFLP. Wang, Sheng-Zheng,Liu, Kai,Si, Ning,Qi, Fang-Jun,Jian, Gui-Liang.

[15]Expression Analysis of Low Temperature Responsive Genes in Eupatorium Adenophorum Spreng Using cDNA-AFLP. Wang, Junying,Zhang, Haiwen,Huang, Rongfeng.

[16]Variations of DNA methylation and genes expression for brown fiber during its development process. Li, Zhenpeng,Li, Tingchun,Fan, Honghong,Guo, Ning,Sun, Xu,Cai, Yongping,Lin, Yi,Li, Zhenpeng,Li, Tingchun.

[17]MOLECULAR ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN BIRCH (BETULA PLATYPHYLLA) INFLORESCENCE. Sun, Sheng,Kang, Xiu-Ping,Zheng, Shao-Wen,Hao, Rui-Jie,Liu, Qun-Long,Zhang, Jian-Cheng,Xing, Guo-Ming,Tian, Yong-Sheng. 2012

[18]Identification of photoperiod-regulated gene in soybean and functional analysis in Nicotiana benthamiana. Sha Ai-Hua,Shan Zhi-Hui,Zhang Xiao-Juan,Wu Xue-Jun,Qiu De-Zheng,Zhou Xin-An,Chen Yin-Hua,Sha Ai-Hua,Shan Zhi-Hui,Zhang Xiao-Juan,Wu Xue-Jun,Qiu De-Zheng,Zhou Xin-An.

[19]Isolation and identification of a gene in response to rice blast disease in rice. Zheng, XW,Chen, XW,Zhang, XH,Lin, ZZ,Shang, JJ,Xu, JC,Zhai, WX,Zhu, LH.

[20]Histological and transcriptional characterization of a novel recessive genic male sterility mutant in sesame (Sesamum indicum L.). Wu, Kun,Liu, Hongyan,Zuo, Yang,Yang, Minmin,Zhao, Yingzhong.

作者其他论文 更多>>

微信小程序