Identification of the Key Transcription Factors Regulating the Expression of the Genes Associated with Barley Malt Quality during Malting

文献类型： 外文期刊

第一作者： Li, Mengdi

作者： Li, Mengdi;Zhang, Guoping;Ye, Lingzhen;Cai, Kangfeng;Zheng, Nannan;Zhang, Guoping;Ye, Lingzhen

作者机构：

关键词： barley (Hordeum vulgare L; ); hydrolyticenzyme; malt quality; transcriptome; WGCNA; PPI analysis

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 21 期

页码：

收录情况： SCI

摘要： Barley malt is produced through amalting process; it begins withsteeping followed by germination and kilning, in which dramatic changeshappen for a large number of physiological and biochemical traitsin barley seeds. The objectives of this study were to comprehensivelyinvestigate the phenotypic changes during malting, and identify thekey regulators that modulate the expression of genes associated withmalt quality traits. The results showed that there was a significantpositive correlation between gibberellic acid (GA) content and theactivities of some hydrolytic enzymes, including alpha-amylases,beta-amylases, and limit dextrinase (LD), and a significant negativecorrelation between GA and beta-glucan content. Starch contenthad little change, but starch granules were pitted severely duringmalting. Weighted gene coexpression analysis (WGCNA) identified thegenes associated with the greatest changes of the examined malt traitsduring malting. The correlation analysis and protein-proteininteraction (PPI) analysis detected several key transcriptional factor(TF) regulating genes associated with malt quality. These genes andTFs regulating malting traits are potentially useful in barley breedingfor malt quality improvement.

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