Identification and Heterologous Expression of a Delta 4-Fatty Acid Desaturase Gene from Isochrysis sphaerica
文献类型: 外文期刊
第一作者: Guo, Bing
作者: Guo, Bing;Jiang, Mulan;Wan, Xia;Gong, Yangmin;Liang, Zhuo;Hu, Chuaniong;Jiang, Mulan;Hu, Chuaniong
作者机构:
关键词: Isochrysis sphaerica;Delta 4-fatty acid desaturase gene;heterologous expression
期刊名称:JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:2.351; 五年影响因子:2.65 )
ISSN: 1017-7825
年卷期: 2013 年 23 卷 10 期
页码:
收录情况: SCI
摘要: The marine microalga Isochrysis sphaerica is rich in the very-long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (EPA, C20:5 omega-3) and docosahexaenoic acid (DHA, C22:6 omega-3) that are important to human health. Here, we report a functional characterization of a Delta 4-fatty acid desaturase gene (FAD4) from I. sphaerica. IsFAD4 contains a 1,284 bp open reading frame encoding a 427 amino acid polypeptide. The deduced amino sequence comprises three conserved histidine motifs and a cytochrome b5 domain at its N-terminus. Phylogenetic analysis indicated that IsFad4 formed a unique Isochrysis clade distinct from the counterparts of other eukaryotes. Heterologous expression of IsFAD4 in Pichia pastoris showed that IsFad4 was able to desaturate docosapentaenoic acid (DPA) to form DHA, and the rate of converting DPA to DHA was 79.8%. These results throw light on the potential industrial production of specific polyunsaturated fatty acids through IsFAD4 transgenic yeast or oil crops.
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